Immunotoxicity of Particulate Lead: In Vitro Exposure Alters Pulmonary Macrophage Tumor Necrosis Factor Production and Activity

Details

• Personal Author:
  Cohen MD ; Yang Z ; Zelikoff JT
• Description:
  Rabbit pulmonary macrophages were exposed in vitro to particulate lead oxide (PbO) for periods of up to 72 h and then assayed for the activity of tumor necrosis factor-alpha (TNFa) released after stimulation with lipopolysaccharide (LPS). The levels of TNFa obtained from PbO-treated cells were decreased in a dose-dependent manner as compared with metal-free control cells for each time point examined. Cells treated simultaneously with both LPS and PbO yielded less monokine than did cells receiving LPS alone. In addition, incubation of cell-free TNFa with PbO resulted in a diminution of cytotoxicity directed against TNFa-sensitive tumor target cells. Macrophage burdens of PbO particles increased with both the length of incubation and concentration of PbO used; increases in cellular lead burdens were paralleled by reductions in cell viability. Thus, under in vitro conditions, PbO affects the levels of the immunoregulatory monokine TNFa and also disrupts its cyto-toxic properties after release from activated macrophages. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Immune System Lead Occupational Health Particulate Matter Safety
• Keywords:
  Cellular Effects Cytotoxic Effects Immunology Immunotoxins In Vitro Studies Particulates Toxic Effects
• ISSN:
  0098-4108
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  377-392
• Volume:
  42
• Issue:
  4
• NIOSHTIC Number:
  nn:20060108
• Citation:
  J Toxicol Environ Health 1994 Aug; 42(4):377-392
• Contact Point Address:
  Dr. Mitchell D. Cohen, Department of Environmental Medicine, New York University Medical Center, Long Meadow Road, Tuxedo, NY 10987, USA
• CAS Registry Number:
  Lead (CAS RN 7439-92-1)
• Federal Fiscal Year:
  1994
• Performing Organization:
  Mount Sinai School of Medicine, New York
• Peer Reviewed:
  True
• Start Date:
  19920701
• Source Full Name:
  Journal of Toxicology and Environmental Health
• End Date:
  19970630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5c07ee5932663498c663f39b9f773ffc76e67a303d9686f6d9b7e0f807bd990976726b4aa8a10a2898819b0aee4a325632677f516f96845d933aa646f0272d49
• Download URL:
  https://stacks.cdc.gov/view/cdc/224123/cdc_224123_DS1.pdf
• File Type:
  [PDF - 1.04 MB ]