Multiple Topical Applications of Arachidonic Acid to Mouse Ears Induce Inflammatory and Proliferative Changes

Details

• Personal Author:
  Beaver TH ; Doherty NS ; Nordlund JJ ; Rheins LA
• Description:
  The response to daily topical applications of arachidonic acid (0.25 - 4 mg/ear/day) to the ears of outbred CD-1 mice was monitored. The first application produced erythema, extravasation of plasma proteins resulting in an increase in ear weight, and some neutrophil accumulation (detected histologically and quantified by myeloperoxidase content). The second application produced minimal edema but did cause erythema and a greater accumulation of neutrophils. Subsequent daily application caused erythema, neutrophil accumulation, and an increase in ear weight predominantly due to cell proliferation (epidermis and connective tissue). Daily applications of other unsaturated fatty acids did not match the response induced by arachidonic acid. Mast cell deficient mice (W/Wv) exhibited a smaller edema response to the first dose of arachidonic acid compared to either their wild-type controls or CD-1 mice. In addition, W/Wv mice exhibited a smaller ear weight increase and myeloperoxidase accumulation following eight daily doses of arachidonic acid. However, epidermal proliferation was similar in all the strains of mice tested. These data suggest that the edema caused by the first topical application of arachidonic acid is partly mast cell mediated. Mast cells also appear to be involved in the neutrophil infiltration induced by multiple topical applications, but not in the epidermal proliferation. [Description provided by NIOSH]
• Subjects:
  Animals Immune System Occupational Health Safety Skin
• Keywords:
  Animal Studies Biological Effects Dermal Absorption Dermal Exposures Immune Reaction Immune System Skin Irritants Skin Sensitivity
• ISSN:
  0022-202X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  298-302
• Volume:
  91
• Issue:
  4
• NIOSHTIC Number:
  nn:20060083
• Citation:
  J Invest Dermatol 1988 Oct; 91(4):298-302
• Contact Point Address:
  Niall S. Doherty Ph.D., Merrell Dow Research Institute, 2110 E. Galbraith Rd., Cincinnati, OH 45215
• CAS Registry Number:
  Arachidonic acid (CAS RN 506-32-1)
• Federal Fiscal Year:
  1989
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19850401
• Source Full Name:
  Journal of Investigative Dermatology
• End Date:
  19910131
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cab8bca9985d33a146aa1bef6e581e3b835534021e74a46bcf0081491beadb43ed993eb15249646ba53272bf6a3e2184d2a57147c3856d030bd3dbfdac8a25cc
• Download URL:
  https://stacks.cdc.gov/view/cdc/224111/cdc_224111_DS1.pdf
• File Type:
  [PDF - 2.15 MB ]