Exposure to Polycyclic Aromatic Hydrocarbons Related to Sperm Quality and DNA Integrity

Details

• Personal Author:
  Jeng A ; Pan C ; Wu M
• Description:
  The objectives of this study were to explore i) whether exposure to polycyclic aromatic hydrocarbons (PAHs) contribute to the alteration of male sperm quality and DNA integrity of coke-oven workers, and ii) whether gene polymorphism can modulate the effect induced by PAHs . Our specific aims were to i) determine the correlation between 16 individual PAH species and their biomarkers and sperm quality and DNA integrity; ii) determine sperm oxidative damage by identifying specific DNA lesions, iii) assess the correlation between gene polymorphisms and sperm quality and DNA integrity. A longitudinal study included repeated measurements to account for PAH exposure as it relates to toxic effects on sperms during spermatogenesis. Personal breathing-zone air samples and urine samples were collected to determine PAH external and internal exposure levels, respectively. Semen was collected to assess sperm quality, DNA adducts, and oxidative damage. Blood samples were collected for genotyping. The high exposed group had a significantly lower percentage of motile, viable and normal morphological sperms as compared to the control. The PAH exposed group experienced higher DNA fragmentation percentages, 8-oxodG concentrations, bulky DNA adducts, and BaP like DNA adducts. The concentration of 1-OHP levels negatively correlated with normal sperm morphology and motility. PAHs with higher molecular weights tend to correlate with sperm quality and DNA fragmentation. GSTM1 null and CYP1A1 Msp1 men had higher sperm DNA fragmentation and 8-oxodG concentration. Exposure to PAHs altered sperm quality and increased oxidative DNA damage. Genetic polymorphisms influence the susceptibility of men to sperm DNA damage related to exposure to PAHs. Research results laid a foundation for future study related to the effects of PAHs on reproductive health and examine gene and environment interaction. [Description provided by NIOSH]
• Subjects:
  Hydrocarbons Men Occupational Exposure Occupational Health Polycyclic Aromatic Hydrocarbons Safety Spermatogenesis Toxicology Urogenital System
• Keywords:
  Coke Oven Workers DNA Damage Occupational Exposure Polycyclic Aromatic Hydrocarbons Reproductive Effects
• ISSN:
  1096-6080
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  126
• NIOSHTIC Number:
  nn:20059283
• Citation:
  Toxicologist 2012 Mar; 126(Suppl 1):507-508
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  Old Dominion University, Norfolk, Virginia
• Peer Reviewed:
  False
• Start Date:
  20090901
• Source Full Name:
  The Toxicologist. Society of Toxicology 51st Annual Meeting and ToxExpo, March 11-15, 2012, San Francisco, California
• Supplement:
  1
• End Date:
  20120831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:dc09dc297da9d733376c6a756537bf3a1087bf95fe76d2e65d9f5605331fe0899fb717a3c6eb4fb5ccf4d9ff506171d8d71230dce5bedf8c9d3cbb0ce9a2702c
• Download URL:
  https://stacks.cdc.gov/view/cdc/223560/cdc_223560_DS1.pdf
• File Type:
  [PDF - 453.42 KB ]