The Circadian Rhythm of the N-Terminus and C-Terminus of the Atrial Natriuretic Factor Prohormone

Details

• Personal Author:
  Bremmer WF ; Bushell DL ; Greco J ; Hrushesky WHM ; Kanabrocki EL ; Kaplan E ; Olwin JH ; Rumbyrt J ; Scheving LE ; Sothern RB ; Sturtevant FM ; Sturtevant RP ; Third JLHC ; Tsai TH ; Vesely DL
• Description:
  Circadian variation in the circulating concentrations of the N-terminal and C-terminal portions of the atrial natriuretic factor prohormone (pro ANF) was evaluated in 8 men, ages 41-47, who have been followed for 19 years with respect to circadian variation in physiological variables including blood pressure and clinical chemistries. The N-terminus of the ANF prohormone contains two peptides consisting of amino acids 1-30 and 31-67 while the C-terminus contains 1 peptide (amino acids 99-126) of this 126 amino acid prohormone which lower blood pressure and have natriuretic properties. To determine if either the N-terminus and/or the C-terminus of the prohormone have a circadian variation in their circulating plasma concentrations these 8 men had blood samples obtained for radiommunoassay every 3 hr during a 24-hr period. Three radiommunoassays which immunologically recognize (1) the whole N-terminus (i.e. amino acids 1-98), (2) the midportion of the N-terminus (amino acids 31-67) and (3) the C-terminus (amino acids 99-126) of the ANF prohormone were utilized. The whole N-terminus, the midportion of the N-terminus which circulates after being proteolytically cleaved from the rest of the N-terminus, and the C-terminus each had a peak circulating concentration between 0400 and 0700 which were significantly (P < 0.001) higher than their concentrations at any other time throughout the 24-hr period. It was concluded that there is a circadian rhythm in both the N-terminus and C-terminus of the ANF prohormone with peak plasma concentrations near the 24-hr nadir in blood pressure suggesting a possible cause for this blood pressure nadir since both the N-terminus and C-terminus of this prohormone contain blood pressure lowering peptides. [Description provided by NIOSH]
• Subjects:
  Circadian Rhythm Endocrine System Men Occupational Health Safety
• Keywords:
  Amino Acids Author Keywords: Atrial Natriuretic Factor Biological Rhythms Blood Pressure Circadian Rhythms Hormone Activity Hormones Immunoassays N-terminal Prohormone Peptides
• ISSN:
  0742-0528
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Arkansas ; Florida ; Illinois ; Minnesota ; New York ; OSHA Region 2 ; OSHA Region 4 ; OSHA Region 5 ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  51-57
• Volume:
  7
• Issue:
  1
• NIOSHTIC Number:
  nn:20059033
• Citation:
  Chronobiol Int 1990 Jan; 7(1):51-57
• Contact Point Address:
  David L. Vesely, J.A. Haley VA Medical Center-151, 13000 Bruce B. Downs Boulevard, Tampa, FL 33612, U.S.A.
• Federal Fiscal Year:
  1990
• Performing Organization:
  University of Arkansas Med Scis Ltl Rock, Little Rock, Arkansas
• Peer Reviewed:
  True
• Start Date:
  19791201
• Source Full Name:
  Chronobiology International
• End Date:
  19880301
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5dc47d5d06143577dda7f3c6553b564189e3e9b2103470fb9ac18637a4d5a58d470440c41e290f4654f4dc7a86404d82df1b4b5c7e725876b714ba0bab0d0558
• Download URL:
  https://stacks.cdc.gov/view/cdc/223364/cdc_223364_DS1.pdf
• File Type:
  [PDF - 823.79 KB ]