Inhalation Co-Exposure to Ultrafine Carbon and Ozone Leads to Significant Pulmonary and Systemic Oxidative Stress

Details

• Personal Author:
  Erdely A ; Goldsmith WT ; Hubczak J ; Hussain S ; Kelly EE ; Kodali VK ; Majumder N ; Mark RA ; Nurkiewicz TR ; Williams XM
• Description:
  Particles and gases are integral components of ambient air pollution and both contribute significantly in adverse health outcomes. Recent epidemiologic data indicates that possible synergistic interactions between these components mediate the adverse phenotypes. Mechanistically, gaseous components can modify the particle surfaces and can reach distal portions of the lung, owing to the deeper penetrability of ultrafine particles. We hypothesize that ultrafine particles of carbon black (CB) and ozone (O3) co-exposure will lead to significantly greater oxidative stress response in the lungs, heart and liver compared with individual/single toxicant exposures. We performed rodent (C57Bl/6J mice) whole-body inhalation exposures (air, CB, O3 or CB+O3) and studied acellular and cellular bimolecular free radical production by immuno-spin trapping. Mice were exposed to 2.0 +/- 0.02 ppm O3 and/or 10 +/- 0.6 mg/m3 CB for 3 hours (16 microg particle deposited dose). Aerosol mobility (140 +/- 2 nm), and aerodynamic (84 +/- 1 nm) diameters were measured by scanning mobility particle analyzer (SMPS 3938) and an electrical low-pressure impactor (ELPI+). Fourier transformed infrared (FTIR) spectroscopy demonstrated significant alteration in particle surface functional group composition after interaction with O3. Ferric reducing ability of serum (FRAS) assay demonstrated significantly greater acellular oxidative potentials for co-exposure aerosol. A significantly greater increase in pulmonary and distal organ free radical production, xanthine oxidase activity and gene expression occurred after co-exposures compared with single exposures. In conclusion, our studies confirm interaction of gaseous and particle components of air pollution. By demonstrating distal organ oxidative stress response after pulmonary exposure, we describe a potential pathophysiologic mechanism for cardiovascular and hepatic dysfunction by air pollution exposure. Further mechanistic studies are underway to elaborate these findings using organoids and disease animal models. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Biological Assay Cardiovascular System Digestive System Digestive System Diseases Environmental Exposure Epidemiology Free Radicals Hazardous Substances Immune System Laboratories Liver Lung Lung Diseases Musculoskeletal System Occupational Health Ozone Particulate Matter Pathology Respiratory System Respiratory Tract Diseases Safety Spectrum Analysis Toxicology

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• Keywords:
  Air Pollutants Bioassays Carbon Black Cardiac Function Cell Alteration Exposure Assessment Exposure Levels Extremities Free Radical Generation Gases Gene Expression Heart Humans Immune Reaction Inhalation Laboratory Animals Laboratory Testing Liver Disorders Liver Function Lung Disorders Lung Function Nanomaterials Nanoparticles Nanotoxicology Oxidative Stress Particle Aerodynamics Particle Diameter Particle Size Particulates Pulmonary System Spectrographic Analysis Surface Properties Synergism Synergistic Effects Toxic Effects Toxic Materials Ultrafine Particles

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  174
• Issue:
  1
• NIOSHTIC Number:
  nn:20058976
• Citation:
  Toxicologist 2020 Mar; 174(1):367
• CAS Registry Number:
  Carbon black (CAS RN 1333-86-4) ; Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  2020
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 59th Annual Meeting and ToxExpo, March 15-19, 2020, Anaheim, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:12f6e425bfd7c1e79bee897249c39f44f0e50367ea5215a9021c8c529ee6e3233c6d5d0831f694515d4c248929e0c716e227041b15b2b4fe31559b5c0f862430
• Download URL:
  https://stacks.cdc.gov/view/cdc/223320/cdc_223320_DS1.pdf
• File Type:
  [PDF - 523.82 KB ]