Aerosol Physicochemical Determinants of Carbon Black and Ozone Inhalation Co-Exposure Induced Pulmonary Toxicity

Details

• Personal Author:
  Amedro J ; Erdely A ; Goldsmith T ; Harkema JR ; Hussain S ; Kelley EE ; Khramtsov VV ; Kodali V ; Majumder N ; Nurkiewicz TR ; Velayutham M
• Description:
  Air pollution accounts for more than 7 million premature deaths worldwide. Using ultrafine carbon black (CB) and ozone (O3) as a model for an environmental co-exposure scenario, the dose response relationships in acute pulmonary injury and inflammation were determined by generating, characterizing, and comparing stable concentrations of CB aerosols (2.5, 5.0, 10.0 mg/m3), O3 (0.5, 1.0, 2.0 ppm) with mixture CB+O3 (2.5 + 0.5, 5.0 + 1.0, 10.0 + 2.0). C57BL6 male mice were exposed for 3 hours by whole body inhalation and acute toxicity determined after 24 h. CB itself did not cause any alteration, however, a dose response in pulmonary injury/inflammation was observed with O3 and CB+O3. This increase in response with mixtures was not dependent on the uptake but due to enhanced reactivity of the particles. Benchmark dose modeling showed several-fold increase in potency with CB+O3 compared to CB or O3 alone. Principal component analysis provided insight into response relationships between various doses and treatments. There was a significant correlation in lung responses with charge-based size distribution, total/alveolar deposition, oxidant generation and antioxidant depletion potential. Lung tissue gene/protein response demonstrated distinct patterns that are better predicted by either particle dose/aerosol responses (IL-1beta, KC, TGF-beta) or particle reactivity (TSLP, IL13, IL-6). Hierarchical clustering showed a distinct signature with high dose and a similarity in mRNA expression pattern of low and medium doses of CB+O3. In conclusion, we demonstrate that the biological outcomes from CB+O3 co-exposure are significantly greater than individual exposures over a range of aerosol concentrations and aerosol characteristics can predict biological outcome. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Immune System Laboratories Occupational Health Ozone Respiratory System Safety Toxicology
• Keywords:
  Air Pollutants Author Keywords: Ozone Carbon Black Co-exposure Exposure Assessment Immune Reaction Inflammation Inhalation Inhalation Studies Laboratory Animals Mixed Exposures Nanoparticles Nanotoxicology Physicochemical Properties Ultrafine Carbon Black Ultrafine Particles

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  61-78
• Volume:
  191
• Issue:
  1
• NIOSHTIC Number:
  nn:20066392
• Citation:
  Toxicol Sci 2023 Jan; 191(1):61-78
• Contact Point Address:
  Salik Hussain, Department of Physiology and Pharmacology, Center for Inhalation Toxicology (iTOX), School of Medicine, West Virginia University, Morgantown, WV 26506, USA
• Email:
  salik.hussain@hsc.wvu.edu
• CAS Registry Number:
  Carbon black (CAS RN 1333-86-4) ; Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  2023
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Toxicological Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:22412dc19b1bf39ab0868dae8cf605a411bd80a555de10f56ec3556c0596360d6c33282eb4a42c3deb016a54b0bb6decd4b2c23a5ae03e786e1cbff5787eefd7
• Download URL:
  https://stacks.cdc.gov/view/cdc/212842/cdc_212842_DS1.pdf
• File Type:
  [PDF - 1.84 MB ]