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Polymorphic N-Acetylation of 2-Aminofluorene by Cell-Free Colon Extracts from Inbred Mice



Details

  • Personal Author:
  • Description:
    The increased risk of rapid acetylator humans for the development of colorectal cancer has created interest in experimental animal models to study the relationship of N-acetyltransferase phenotype to colon cancer. Colon cytosols from inbred mouse lines were assayed for the ability to N-acetylate 2-aminofluorene to determine if the mouse model of the N-acetyltransferase polymorphism could be used to study this relationship. The results indicate that the colon acetylcoenzyme A: 2-aminofluorene- N-acetyltransferase activity parallels that of the liver. Colon activity from slow acetylator (A and B6.A) mouse lines is significantly lower than that of rapid acetylator (B6, B6.D, and A.B6) lines. p-Aminobenzoic acid N-acetyltransferase activity also differed between colon cytosols from rapid and slow acetylator strains. Isoniazid acetylation in colon and in liver did not differ between phenotypes. Northern blot analysis demonstrated the presence of mRNA for both NAT-1 and NAT-2 in mouse colon as well as in mouse liver. These results indicate that the N-acetyltransferase polymorphism is expressed in mouse colon when 2-aminofluorene or p-aminobenzoic acid is used as substrate and therefore the mouse may be a model for study of the effect of acetylator phenotype on development of colorectal cancer in humans. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0960-314X
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Pages in Document:
    71-76
  • Volume:
    3
  • Issue:
    2
  • NIOSHTIC Number:
    nn:20058973
  • Citation:
    Pharmacogenetics 1993 Apr; 3(2):71-76
  • Contact Point Address:
    Gerald N. Levy, Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA
  • Federal Fiscal Year:
    1993
  • Performing Organization:
    University of Michigan at Ann Arbor, Ann Arbor, Michigan
  • Peer Reviewed:
    True
  • Start Date:
    19900301
  • Source Full Name:
    Pharmacogenetics
  • End Date:
    19940228
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:07db0cd418137f9919ea525da650bb2120b6aa93f3c2192410251319835770d1fd0a30b5b7e35e464abbb01470bff8b5b565db77f69fc4bd112d285e99660c98
  • Download URL:
  • File Type:
    Filetype[PDF - 626.38 KB ]
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