Disruption of Bronchial Cell Monolayer Integrity by Organomodified Nanoclays and Their Incinerated Byproducts

Details

• Personal Author:
  Dinu C ; Gupta R ; Rojanasakul Y ; Stueckle, Todd A. ; Wagner A ; White A
• Description:
  Organomodified nanoclays (ONCs) represent one of the most used engineered nanomaterials (ENMs) as nanofiller in emerging advanced manufacturing strategies to produce a diverse number of polymer nanocomposites. Different organic quaternary ammonium coatings on these 2-dimensional montmorillonite nanoclays allow for their incorporation in novel or replacement technologies in thin-film, aerospace, automobile, consumer, and health care polymer nanocomposite applications. Compared with other ENMs, little information exists on risks to occupational pulmonary health along the ONC life cycle that encompass synthesis, handling, manipulation, and disposal. This study hypothesized that coating type, incineration status, and time-dependent effects of ONC exposure would impact bronchial epithelial cell monolayer integrity, a key target following inhalation exposure. Highthroughput in vitro screening strategies including high content imaging, electric cell impedance sensing, and flow cytometry were employed to evaluate a set of pre- and post-incinerated ONCs for acute effects and fate of the monolayer post-exposure. Using each particle's IC(50) cell viability in a BEAS-2B cell model, pristine nanoclay exposure caused acute loss of monolayer integrity, decreased metabolism, and increased apoptosis. Three different ONCs, however, displayed minimal loss to monolayer integrity despite coating type-dependent differences in apoptosis induction and decreased cell metabolism. Conversely, incinerated nanoclay byproducts caused decreased monolayer integrity, increased cell necrosis, and little evidence for reestablishment of the epithelial monolayer. These results suggest the type of quaternary ammonium coating and incineration status largely impacts mechanism of cytotoxicity, cell metabolism, and the recovery ability of the exposed bronchial epithelial cell monolayer. An integrated high-throughput in vitro screening strategy, using high content imaging and traditional in vitro methods, represents a rapid pulmonary epithelial toxicity assessment approach to prioritize ENMs for further evaluation and serves to inform 'prevention-by-design' material development strategies. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Cytotoxins Hazardous Substances Lung Minerals Occupational Health Polymers Respiratory System Safety Toxicology
• Keywords:
  Acute Toxicity Advanced Manufacturing Advanced Materials Ammonium Compounds Cell Cultures Cell Culture Techniques Cell Death Cell Metabolism Cellular Effects Coatings Cytotoxicity Exposure Assessment Incineration Inhalation In Vitro Study Lung Cells Molecular Structure Nanoclays Nanocomposites Nanomaterials Nanotoxicology Prevention Through Design PTD Screening Methods Toxic Materials

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  269-270
• Volume:
  174
• Issue:
  1
• NIOSHTIC Number:
  nn:20058957
• Citation:
  Toxicologist 2020 Mar; 174(1):269-270
• Federal Fiscal Year:
  2020
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 59th Annual Meeting and ToxExpo, March 15-19, 2020, Anaheim, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1cfdfbb439dc6a42b33289b2abed1672bef794d1de637dd5b2ba4b795029948b9b9806e1323e09c8138b3d3059d6981a453518e155cf551bd062ef9c5e640c36
• Download URL:
  https://stacks.cdc.gov/view/cdc/223305/cdc_223305_DS1.pdf
• File Type:
  [PDF - 541.20 KB ]