The Relationship Between Urinary 1-Hydroxypyrene and DNA Adducts in the Exfoliated Bladder Cells of Firefighters

Details

• Personal Author:
  Jackson MV
• Description:
  During this study, firefighters were examined based on their potential exposure to polycyclic aromatic hydrocarbons. Both pre-shift and post-shift urine samples were analyzed by measuring 1-hydroxypyrene levels and DNA adducts in the exfoliated bladder cells. 23 non clean catch urine samples were collected from 15 different firefighters, with 10 of the samples being post-shift samples. We hypothesized that firefighters who had been exposed to PAH will have an increased level of 1-Hydroxypyrene and DNA adducts in their bladder cells. 1-HP levels along with DNA adducts in exfoliated bladder cells were compared between pre-shift and post-shift samples. 1-HP levels were measured using the Jongeneelen et al. method. DNA was first isolated using the Wizard Genomic DNA Kit, and adduct levels were quantified by 32P-post labeling adenosine 5'triphosphate excess thin layer chromatography. When comparing pre- and post-shift samples, three out of four were found to have an increase in 1-HP. The pre- shift samples 1-hydroxypyrene mean values was determined to be 0.48053, while the post-shift mean 1-hydroxypyrene values were 0.9736. The increase between pre- and post-shift 1-hydroxypyrene levels indicates a small exposure to PAH. Although all but one sample fell below the 1-HP ACGIH BEI value of 1 ug/l, the data suggests that these firefighters are exposed to PAH. A Pearson correlation coefficient was generated comparing 1-Hp levels and DNA adducts and a negative correlation was reported. [Description provided by NIOSH]
• Subjects:
  Biomarkers Emergency Responders Firefighters Hydrocarbons Occupational Exposure Occupational Health Polycyclic Aromatic Hydrocarbons Safety
• Keywords:
  Bladder Cancer DNA Adducts Exposure Assessment Fire Fighters First Responders Occupational Exposure Polycyclic Aromatic Hydrocarbons
• Publisher:
  University of Cincinnati
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Thesis
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-26
• NIOSHTIC Number:
  nn:20058772
• Citation:
  Cincinnati, OH: University of Cincinnati, 2011 Nov; :1-26
• CAS Registry Number:
  1-Hydroxypyrene (CAS RN 5315-79-7)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  False
• Start Date:
  20090901
• Source Full Name:
  The relationship between urinary 1-hydroxypyrene and DNA adducts in the exfoliated bladder cells of firefighters
• End Date:
  20120831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5c6352dd8d472f30ca2bef2a2a27312953108da8536df4623a6b4d296a22db372ae8d1038dee9650373a25bbc054b6e28f62ef1e804735088e64eb68bf6daaf8
• Download URL:
  https://stacks.cdc.gov/view/cdc/223170/cdc_223170_DS1.pdf
• File Type:
  [PDF - 320.77 KB ]