Determination of Polycyclic Aromatic Hydrocarbon Levels in German Tunnel Workers Exposed to Diesel Exhaust

Details

• Personal Author:
  Boachie FK ; Genter MB ; Succop P ; Talaska G
• Description:
  Diesel exhaust emissions include levels of volatile organic compounds and particulates that can be harmful to the health of both humans and the environment. Polycyclic aromatic hydrocarbons (PAH) are just one of these particulates that can be absorbed through dermal contact, inhalation, or ingestion. Exposures to excess occupational amounts of diesel exhaust have been linked to an increased risk of lung cancer; however, research supporting to possibility of a connection between diesel exhaust and bladder cancer has yet to be agreed upon. Objective: The current study examines the validity of using urinary 1-hydroxypyrene (1-HP) as a biomarker of exposure for diesel exhaust by determining a statistical association between levels of 1-HP and the presence of DNA adducts due to their necessity for tumor induction. Method: Thirty urine samples from German tunnel workers were measured by analyzing the levels of 1-HP in worker's pre- and post-shift urine samples over a six week period. 1-hydroxypyrene was detected using HPLC fluorescence techniques. DNA adduct levels were measured in exfoliated urothelial cells using a 32P-postlabelling assay. Each sample was coded and was only revealed after the analyses were complete. Results: The values of 1-HP in urine were consistently higher in post-shift samples although none of the values were above the American Conference of Governmental Industrial Hygienists (ACGIH(R)) level of 1 ug/L. Tobacco smoking had a noted effect on the levels of DNA adducts but there was no observed statistically significant difference in 1-HP values. There was a positive correlation between levels of 1-HP and DNA adducts; however, the results were not deemed statistically significant. Although there were trends observed between 1-HP and DNA adduct levels, the lack of statistical power calls for further research in this area. [Description provided by NIOSH]
• Subjects:
  Biomarkers Construction Industry Hydrocarbons Occupational Exposure Occupational Health Polycyclic Aromatic Hydrocarbons Safety
• Keywords:
  Bladder Cancer Construction Workers Diesel Exhaust DNA Adducts Exposure Assessment Occupational Exposure Polycyclic Aromatic Hydrocarbons Tunnel Workers
• Publisher:
  American Industrial Hygiene Association
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20058771
• Citation:
  AIHce 2012: American Industrial Hygiene Conference and Exposition, June 16-21, 2012, Indianapolis, Indiana. Falls Church, VA: American Industrial Hygiene Association, 2012 Jun; :145
• CAS Registry Number:
  1-Hydroxypyrene (CAS RN 5315-79-7)
• Federal Fiscal Year:
  2012
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  False
• Start Date:
  20090901
• Source Full Name:
  AIHce 2012: American Industrial Hygiene Conference and Exposition, June 16-21, 2012, Indianapolis, Indiana
• End Date:
  20120831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d6b5b69337b7874529d66d344f4488b948fb52b7e183028a8f53769f10c95b643b625443ab12b1c1a5af84ab8c2d1a7afa3d3bea0bef5536594ef024dc7c7d44
• Download URL:
  https://stacks.cdc.gov/view/cdc/223169/cdc_223169_DS1.pdf
• File Type:
  [PDF - 155.04 KB ]