Stability of Lipids in Plasma and Serum: Effects of Temperature-Related Storage Conditions on the Human Lipidome

Details

• Personal Author:
  Barr JR ; Drew NM ; Ivanova AA ; Kuklenyik Z ; Pirkle JL ; Rees JC ; Reis GB
• Description:
  Large epidemiological studies often require sample transportation and storage, presenting unique considerations when applying advanced lipidomics techniques. The goal of this study was to acquire lipidomics data on plasma and serum samples stored at potential preanalytical conditions (e.g., thawing, extracting, evaporating), systematically monitoring lipid species for a period of one month. Split aliquots of 10 plasma samples and 10 serum samples from healthy individuals were kept in three temperature-related environments: refrigerator, laboratory benchtop, or heated incubator. Samples were analyzed at six different time points over 28 days using a Bligh & Dyer lipid extraction protocol followed by direct infusion into a lipidomics platform using differential mobility with tandem mass spectrometry. The observed concentration changes over time were evaluated relative to method and inter-individual biological variability. In addition, to evaluate the effect of lipase enzyme levels on concentration changes during storage, we compared corresponding fasting and post-prandial plasma samples collected from 5 individuals. Based on our data, a series of low abundance free fatty acid (FFA), diacylglycerol (DAG), and cholesteryl ester (CE) species were identified as potential analytical markers for degradation. These FFA and DAG species are typically produced by endogenous lipases from numerous triacylglycerols (TAGs), and certain high abundance phosphatidylcholines (PCs). The low concentration CEs, which appeared to increase several fold, were likely mass-isobars from oxidation of other high concentration CEs. Although the concentration changes of the high abundant TAG, PC, and CE precursors remained within method variability, the concentration trends of FFA, DAG, and oxidized CE products should be systematically monitored over time to inform analysts about possible pre-analytical biases due to degradation in the study sample sets. [Description provided by NIOSH]
• Subjects:
  Laboratories Lipids Occupational Health Quality Control Safety Temperature
• Keywords:
  Author Keywords: Lipidomics Biological Materials Cholesteryl Ester Degradation Fatty Acids Hydrolysis Laboratory Testing Oxidation Quality Control Sample Preparation Stability Temperature Effects Triglycerides

  More +
• ISSN:
  2667-145X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; Ohio ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DSI - Division of Science Integration
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  34-42
• Volume:
  22
• NIOSHTIC Number:
  nn:20064384
• Citation:
  J Mass Spectrom Adv Clin Lab 2021 Nov; 22:34-42
• Contact Point Address:
  Zsuzsanna Kuklenyik, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA, United States
• Email:
  czk9@cdc.gov
• Federal Fiscal Year:
  2022
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Mass Spectrometry and Advances in the Clinical Lab
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fdd72a5af5ddd4dd35e9af9f15b60de037aad4124a97c4453df3ad0617ad1b8b43e6cf3343c6fc01d880d0369f2efbe4f929238704c9e27e77a3587cfe703087
• Download URL:
  https://stacks.cdc.gov/view/cdc/222851/cdc_222851_DS1.pdf
• File Type:
  [PDF - 2.41 MB ]