Dermal Exposure to the Immunomodulatory Antimicrobial Chemical Triclosan Alters the Skin Barrier Integrity and Microbiome in Mice

Details

• Personal Author:
  Anderson, Stacey E. ; Baur R ; Gandhi J ; Hu G ; Lukomska E ; Marshall NB ; Shane HL ; Weatherly LM
• Description:
  Triclosan is an antimicrobial chemical used in healthcare settings that can be absorbed through the skin. Exposure to triclosan has been positively associated with food and aeroallergy and asthma exacerbation in humans and, although not directly sensitizing, has been demonstrated to augment the allergic response in a mouse model of asthma. The skin barrier and microbiome are thought to play important roles in regulating inflammation and allergy and disruptions may contribute to development of allergic disease. To investigate potential connections of the skin barrier and microbiome with immune responses to triclosan, SKH1 mice were exposed dermally to triclosan (0.5-2%) or vehicle for up to 7 consecutive days. Exposure to 2% triclosan for 5-7 days on the skin was shown to increase transepidermal water loss levels. Seven days of dermal exposure to triclosan decreased filaggrin 2 and keratin 10 expression, but increased filaggrin and keratin 14 protein along with the danger signal S100a8 and interleukin-4. Dermal exposure to triclosan for 7 days also altered the alpha and beta diversity of the skin and gut microbiome. Specifically, dermal triclosan exposure increased the relative abundance of the Firmicutes family, Lachnospiraceae on the skin but decreased the abundance of Firmicutes family, Ruminococcaceae in the gut. Collectively, these results demonstrate that repeated dermal exposure to the antimicrobial chemical triclosan alters the skin barrier integrity and microbiome in mice, suggesting that these changes may contribute to the increase in allergic immune responses following dermal exposure to triclosan. [Description provided by NIOSH]
• Subjects:
  Hypersensitivity Immune System Laboratories Occupational Health Safety Skin
• Keywords:
  Allergic Reactions Antimicrobials Author Keywords: Triclosan Barrier Function Dermal Exposures Exposure Assessment Gene Expression Immune Reaction Laboratory Animals Microbiome Skin Skin Exposure

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  223-235
• Volume:
  184
• Issue:
  2
• NIOSHTIC Number:
  nn:20064257
• Citation:
  Toxicol Sci 2021 Dec; 184(2):223-235
• Contact Point Address:
  Rachel Baur, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1000 Frederick Lane, Morgantown, WV 26508, USA
• Email:
  oee6@cdc.gov
• CAS Registry Number:
  Triclosan (CAS RN 3380-34-5)
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Healthcare and Social Assistance ; Oil and Gas Extraction
• Peer Reviewed:
  True
• Source Full Name:
  Toxicological Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4a8c58c01864b04d9505ed343a4aae44d263035ebc37800873adf747d2c88c57fce3ef167ac895d28fc3da9c7b452d80216d150bfbaa9247dc3e96747cb5c70a
• Download URL:
  https://stacks.cdc.gov/view/cdc/222756/cdc_222756_DS1.pdf
• File Type:
  [PDF - 826.32 KB ]