Assessment of Air Sampling Methods and Size Distribution of Virus-Laden Aerosols in Outbreaks in Swine and Poultry Farms

Details

• Personal Author:
  Alba A ; Alonso C ; Davies PR ; Goyal S ; Olson, B. A. ; Raynor PC ; Torremorell M
• Description:
  Swine and poultry viruses, such as porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and highly pathogenic avian influenza virus (HPAIV), are economically important pathogens that can spread via aerosols. The reliability of methods for quantifying particle-associated viruses as well as the size distribution of aerosolized particles bearing these viruses under field conditions are not well documented. We compared the performance of 2 size-differentiating air samplers in disease outbreaks that occurred in swine and poultry facilities. Both air samplers allowed quantification of particles by size, and measured concentrations of PRRSV, PEDV, and HPAIV stratified by particle size both within and outside swine and poultry facilities. All 3 viruses were detectable in association with aerosolized particles. Proportions of positive sampling events were 69% for PEDV, 61% for HPAIV, and 8% for PRRSV. The highest virus concentrations were found with PEDV, followed by HPAIV and PRRSV. Both air collectors performed equally for the detection of total virus concentration. For all 3 viruses, higher numbers of RNA copies were associated with larger particles; however, a bimodal distribution of particles was observed in the case of PEDV and HPAIV. [Description provided by NIOSH]
• Subjects:
  Aerosols Communicable Diseases Environmental Monitoring Food Industry Livestock Occupational Health Particulate Matter Safety Viral Diseases
• Keywords:
  Air Sampler Air Sampling Techniques Animal Viruses Author Keywords: Aerosols Cascade Impactor Disease Transmission Infectious Diseases Livestock Industry Particle Size Pathogens Poultry Industry Viral Diseases

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• ISSN:
  1040-6387
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  298-304
• Volume:
  29
• Issue:
  3
• NIOSHTIC Number:
  nn:20064099
• Citation:
  J Vet Diagn Invest 2017 May; 29(3):298-304
• Contact Point Address:
  Montserrat Torremorell, Department of Veterinary Population Medicine, 385 ASVM, 1988 Fitch Avenue, College of Veterinary Medicine, University of Minnesota-Twin Cities, St. Paul MN 55108
• Email:
  torr0033@umn.edu
• Federal Fiscal Year:
  2017
• Performing Organization:
  University of Minnesota Twin Cities
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of Veterinary Diagnostic Investigation
• End Date:
  20250630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:80f004d2c3d9243afff395812ba3e2f9e054dbf15f96a774db92629c21b0b95d3ca0131a7813fb4dacdf1939204484869212c702a2fd612e45e696714e8ab106
• Download URL:
  https://stacks.cdc.gov/view/cdc/222618/cdc_222618_DS1.pdf
• File Type:
  [PDF - 311.95 KB ]