Feasibility of a High-Volume Filter Sampler for Detecting SARS-CoV-2 RNA in COVID-19 Patient Rooms

Details

• Personal Author:
  Jones RM ; Kaur K ; Kelly KE ; Wilson AM
• Description:
  Aerosolization of SARS-CoV-2 by COVID-19 patients can put healthcare workers and susceptible individuals at risk of infection. Air sampling for SARS-CoV-2 has been conducted in healthcare settings, but methods vary widely and there is need for improvement. The objective of this study was to evaluate the feasibility of using a high-volume filter sampler, BioCapture z720, to detect SARS-CoV-2 in COVID-19 patient rooms in a medical intensive care unit, a dedicated COVID-19 ward, and at nurses' stations. In some locations, the BioSpot-VIVAS, known for high efficiency in the collection of virus-containing bioaerosols, was also operated. The samples were processed for SARS-CoV-2 RNA with multi-plex nested polymerase chain reaction. One of 28 samples collected with the high-volume filter sampler was positive for SARS-CoV-2; all 6 samples collected with BioSpot-VIVAS were negative for SARS-CoV-2. The high-volume filter sampler was more portable and less intrusive in patient rooms than the BioSpot-VIVAS, but limits of detection remain unknown for this device. This study will inform future work to evaluate the reliability of these types of instruments and inform best practices for their use in healthcare environments for SARS-CoV-2 air sampling. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Communicable Diseases Environmental Monitoring Health Care Facilities Workforce And Services Hospitals Occupational Health Safety Viral Diseases
• Keywords:
  Airborne Particles Air Sampling Author Keywords: Airborne Bioaerosols Coronavirus COVID-19 Environmental Monitoring Filters Health Care Facilities Hospital Limit Of Detection LOD Samplers SARS-CoV-2 Viral Diseases Virus Sampling

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• ISSN:
  2398-7308
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 8 ; Utah
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  276-280
• Volume:
  66
• Issue:
  2
• NIOSHTIC Number:
  nn:20063995
• Citation:
  Ann Work Expo Health 2022 Mar; 66(2):276-280
• Contact Point Address:
  Rachael M. Jones, Department of Family & Preventive Medicine, University of Utah, Salt Lake City, UT, USA
• Email:
  rachael.jones@utah.edu
• Federal Fiscal Year:
  2022
• Performing Organization:
  University of Utah
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Annals of Work Exposures and Health
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cf3b28a3d54235d629429e43fdf50c56cbb0f5e33343ae6fc68d0b670c453ebffa59361c7b6dbbbb6da4ab2e01af578bbb348a3e448d86f600b11a81d1f216d6
• Download URL:
  https://stacks.cdc.gov/view/cdc/222524/cdc_222524_DS1.pdf
• File Type:
  [PDF - 166.52 KB ]