Localization of Glutamate Cysteine Ligase Subunit mRNA Within the Rat Ovary and Relationship to Follicular Apoptosis

Details

• Personal Author:
  Diaz D ; Faustman EM ; Kavanagh TJ ; Luderer U
• Description:
  Ovarian levels of the antioxidant tripeptide glutathione (GSH) increase following gonadotropin administration, suggesting that GSH synthesis in the ovary may be associated with follicular growth. In situ hybridization with 35S-labeled riboprobes was used to localize ovarian mRNA expression of the catalytic and modulatory subunits of glutamate cysteine ligase (Gclc and Gclm), the rate-limiting enzyme in GSH synthesis, during each stage of the rat estrous cycle. Gclm was highly expressed in the granulosa cells and oocytes of healthy, growing follicles, not in atretic follicles. Gclc was also highly expressed in follicles; however, unlike Gclm, Gclc was also expressed in corpora lutea and interstitial cells. In a subsequent experiment, the hypothesis that GSH synthesis occurs in healthy, but not in apoptotic, follicles was tested. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to detect apoptotic cells in the ovaries, and in situ hybridization for Gclm and Gclc was performed in adjacent sections of the same ovaries. TUNEL staining was found to be significantly associated with absence of Gclm hybridization in granulosa cells and oocytes and with lack of strong Gclc hybridization in granulosa cells. These results suggest that follicular apoptosis may be associated with down-regulation of Gclm and Gclc transcription in granulosa cells and oocytes. [Description provided by NIOSH]
• Subjects:
  Animals Antioxidants Occupational Health Safety Urogenital System
• Keywords:
  Animal Studies Atresia Author Keywords: Follicle Cell Function Estrous Cycle Gamma-glutamylcysteine Synthetase Glutamates Glutathione Reproductive Effects Ribonucleic Acids RNA

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• ISSN:
  1040-452X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 10 ; OSHA Region 9 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  254-261
• Volume:
  65
• Issue:
  3
• NIOSHTIC Number:
  nn:20058195
• Citation:
  Mol Reprod Dev 2003 Jul; 65(3):254-261
• Contact Point Address:
  Dr. Ulrike Luderer, Center for Occupational and Environmental Health, University of California Irvine, 5201 California Avenue, Suite 100, Irvine, CA 92612
• Email:
  uluderer@uci.edu
• CAS Registry Number:
  Glutathione (CAS RN 70-18-8)
• Federal Fiscal Year:
  2003
• Performing Organization:
  University of California, School of Public Health, Los Angeles, CA
• Peer Reviewed:
  True
• Start Date:
  19990701
• Source Full Name:
  Molecular Reproduction and Development
• End Date:
  20040630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:12a2ce5717b120305f003ef7d1239d04ff8d2bdf131e07341313d115ed910dd94b96f5a151c9039cf099e633a7bbf24b80607eb7221711ae5177a23ef32a3552
• Download URL:
  https://stacks.cdc.gov/view/cdc/221330/cdc_221330_DS1.pdf
• File Type:
  [PDF - 512.98 KB ]