Glove Permeation and Determination of Benomyl in Benlate 50% WP Pesticide Formulation by GC-MS and GC-EC

Details

• Personal Author:
  Que Hee SS ; Zainal H
• Description:
  Benomyl is the active ingredient of the pesticide, Benlate 50% WP. Long-term human skin exposure to Benlate 50% WP causes skin irritation. Benomyl is a suspected carcinogen. The aim of this study was to investigate the protectiveness of gloves used by farm workers, pesticide sprayers, and formulators. A sensitive analytical technique was first developed to determine benomyl. A mass of 10 mg of benomyl was dissolved in 10 mL methanol. One mL of this solution was evaporated to dryness under a stream of nitrogen. A volume of 0.5 mL of acetonitrile was added, followed by 20 uL of diisopropyl ethyl amine and 20 uL of pentafluorobenzyl bromide. After vortexing and heating at 60 degrees C for 16 hours, evaporation under nitrogen, 0.1 mL water was added. Extraction with isooctane (5x0.5 mL) followed by reducing the volume of the combined extracts to 0.5 mL. On gas chromatographic-mass spectrometric analysis, m/z values of 551,492,292, and 181 were observed at a retention time of 41 minutes. This confirmed the formation of di-pentafluorobenzyl carbendazim. Treatment with acetonitrile at 60 degrees C caused the degradation of benomyl to carbendazim that was then derivatized. The base peak at m/z = 492 was used for selected ion quantitation. This resulted in an LQL of 6 ng and a linear range of 10-200 ng. In contrast gas chromatography-electron capture at the same chromatographic conditions resulted in an LQL of 2 ng and a linear range of 4-60 pg. Thus chromatography-electron capture was used as the method of choice. The permeation of benomyl through "SAFESKIN" nitrile glove material using ASTM typeI-PTC 600 permeation cell that employed isopropanol as collection fluid and a challenge solution of 1.2 mg/ mL suspension of benlate 50% WP in water was <100 pg /cm2/min. [Description provided by NIOSH]
• Subjects:
  Farmers Insecticides Nitriles Occupational Health Personal Protective Equipment Protective Clothing Safety Skin
• Keywords:
  Benomyl Farm Workers Gloves Hand Protection Pesticides And Agricultural Chemicals Skin Exposure Sprays
• Publisher:
  American Industrial Hygiene Association
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  34
• NIOSHTIC Number:
  nn:20058112
• Citation:
  American Industrial Hygiene Conference and Exposition, June 1-6, 2002, San Diego, California. Fairfax, VA: American Industrial Hygiene Association, 2002 Jun; :34
• CAS Registry Number:
  Benomyl (CAS RN 17804-35-2)
• Federal Fiscal Year:
  2002
• Performing Organization:
  University of California Los Angeles
• Peer Reviewed:
  False
• Start Date:
  20000630
• Source Full Name:
  American Industrial Hygiene Conference and Exposition, June 1-6, 2002, San Diego, California
• End Date:
  20040531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b2e77e07cecb341b9fc9588ce6991781134adf9b52e6a1b44d0287edacb3853b60b248085e1c41801fb36ba1267758bbed66f1e04969453855f2be8b4806416d
• Download URL:
  https://stacks.cdc.gov/view/cdc/221258/cdc_221258_DS1.pdf
• File Type:
  [PDF - 2.76 MB ]