Volatile Metabolites Produced by Three Strains of Stachybotrys chartarum Cultivated on Rice and Gypsum Board

Details

• Personal Author:
  Gao, Peng-Fei ; Martin, Jennifer E.
• Description:
  Stachybotrys chartarum (atra) is a toxigenic fungus frequently found in water-damaged buildings. Although microbial volatile organic compounds (MVOCs) produced by Aspergillus, Penicillium, and other fungi have been investigated extensively, little information exists on what MVOCs can be produced by S. chartarum. In this study, three strains of S. chartarum isolated from water-damaged residential homes in Cleveland, Ohio, were cultivated on rice and gypsum board. Air samples were collected after one, two, three, four, and six weeks of cultivation using Tenax TA tubes. Unique MVOCs were determined and other alcohols, ketones, and terpenes were also investigated using gas chromatography/mass spectrometry after thermal desorption from the sampling tube. Four unique MVOCs, 1-butanol, 3-methyl-1-butanol, 3-methyl-2-butanol, and thujopsene, were detected on rice cultures, and only one of them (1-butanol) was detected on gypsum board cultures. For a given strain, volatiles were considerably different with different cultivation media. Concentration profiles of the volatile compounds varied among compounds; however,each compound exhibited corresponding concentration trends between the strains. In comparison with our previous studies of five Aspergillus species on gypsum board under the same experimental conditions, fewer unique MVOCs were produced by S. chartarum, and they were quite different. It thus may be possible to use marker-unique MVOCs as a fingerprint to distinguish fungi in indoor environments once enough information becomes available. Our findings also indicate that volatiles produced by S. chartarum may represent a relatively small fraction of the total volatiles present in problem buildings where Aspergillus spp., Penicillium spp., and other fungi usually coexist. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollution, Indoor Biological Products Energy Metabolism Environmental Health Environmental Monitoring Fungi Hazardous Substances Mass Spectrometry Microbiology Occupational Health Safety

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• Keywords:
  Air-samples Author Keywords: Volatile Metabolites Biological-material Cultures Gas-chromatography Indoor-air-pollution Indoor-environmental-quality Mass-spectrometry Metabolites Microorganisms Organic-compounds Sampling Stachybotrys Atra Stachybotrys Chartarum Toxic-materials Unique MVOCs Volatiles Water-Damaged Buildings

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• Series:
  Mining Publications
• ISSN:
  1047-322X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  PRL - Pittsburgh Research Laboratory ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  430-436
• Volume:
  17
• Issue:
  6
• NIOSHTIC Number:
  nn:20022270
• Citation:
  Appl Occup Environ Hyg 2002 Jun; 17(6):430-436
• Contact Point Address:
  Pengfei Gao, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV
• CAS Registry Number:
  1-Butanol (CAS RN 71-36-3) ; Isoamyl alcohol (CAS RN 123-51-3)
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Applied Occupational and Environmental Hygiene
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5048c9fde2f637b9d82185dbe4612d68f2e1d6b415ed78a3bc0eb727ad76877f3217b4636f49f0822e4f3a1c39af50605c885d48223f9a316d76d3d8c8f97e3c
• Download URL:
  https://stacks.cdc.gov/view/cdc/220545/cdc_220545_DS1.pdf
• File Type:
  [PDF - 698.11 KB ]