An Improved Method for the Direct Determination of Cadmium in Biological Materials by Atomic Absorption Spectrophotometry

Details

• Personal Author:
  Blackstone M ; Richdale N
• Description:
  A method was presented for the determination of metals in mammalian tissues using an atomic absorption method. The procedure calls for digestion of the sample with a quaternary ammonium-hydroxide. With this method one can measure metals which are normally present in low concentrations such as cadmium (7440439) and nickel (7440020) with significant sensitivity and a minimal amount of sample handling. Two month old rats were exposed through inhalation to cadmium-oxide (1306190) aerosols daily for 8 hour periods. The precision of the method was determined in repeated analyses of control and exposed lung tissue. All lungs were divided into approximately five equal portions and each portion analyzed for cadmium, nickel, and zinc (7440666). To determine cadmium and nickel concentrations, 200 to 300 milligrams of wet tissue are needed for precision. These weights can be reduced to 1/20 for the analysis of zinc and copper (7440508). The absorbed amounts were small, suggesting that if the cadmium-oxide aerosol was absorbed, it was also effectively cleared from the system. [Description provided by NIOSH]
• Subjects:
  Cadmium Laboratories Nickel Occupational Health Respiratory System Safety
• Keywords:
  Analytical-methods Cadmium-compounds Grants-other Inhalation-studies Laboratory-animals Nickel-compounds NIOSH-Grant
• Series:
  Grant Final Reports
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Final Grant Report
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OEP - Office of Extramural Programs
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-13
• NIOSHTIC Number:
  nn:00190684
• NTIS Accession Number:
  PB90-129438
• Citation:
  Kettering Laboratory, Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, Ohio :1-13
• Contact Point Address:
  Environmental Health University of Cincinnati Eden and Bethesda Avenues Cincinnati, Ohio 45219
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9) ; Cadmium oxide (CAS RN 1306-19-0) ; Copper (CAS RN 7440-50-8) ; Nickel (CAS RN 7440-02-0) ; Zinc (CAS RN 7440-66-6)
• NORA Priority Area:
  Grants Other
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  False
• Start Date:
  19710401
• Source Full Name:
  Kettering Laboratory, Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, Ohio
• End Date:
  19760531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8974e3f9f2edd6baa77e4eb1751d5e433dd79cd8b9a3ca9d47ca6f6342f9b60bda34e65f409fc14abdf9e5db813b5458377429f622afff4b7867b6399fa87ff9
• Download URL:
  https://stacks.cdc.gov/view/cdc/217768/cdc_217768_DS1.pdf
• File Type:
  [PDF - 406.05 KB ]