Ovalbumin-Sensitized Mice Have Altered Airway Inflammation to Agriculture Organic Dust

Details

• Personal Author:
  Dickinson JD ; Nelson AJ ; Poole JA ; Romberger DJ ; Warren KJ ; Wyatt TA
• Description:
  Agriculture exposures are associated with reducing the risk of allergy and asthma in early life; yet, repeated exposures later in life are associated with chronic bronchitis and obstructive pulmonary diseases. The objective of this study was to investigate the airway inflammatory response to organic dust extract (ODE) in mice with established ovalbumin (OVA)-induced experimental asthma. C57BL/6 mice were either OVA sensitized/aerosol-exposed or saline (Sal) sensitized/aerosol-challenged. Both groups were then subsequently challenged once with intranasal saline or swine confinement ODE to obtain 4 treatment groups of Sal-Sal, Sal-ODE, OVA-Sal, and OVA-ODE. Airway hyper-responsiveness (AHR) to methacholine, bronchiolar lavage fluid, lung tissues, and serum were collected. Intranasal inhalation of ODE in OVA-treated (asthmatic) mice (OVA-ODE) increased AHR and total cellular influx marked by elevated neutrophil and eosinophil counts. Flow cytometry analysis further demonstrated that populations of CD11chi dendritic cells (DC), CD3+ T cells, CD19+ B cells, and NKp46+ group 3 innate lymphoid cells (ILC3) were increased in lavage fluid of OVA-ODE mice as compared to ODE or OVA alone. Alveolar macrophages, DC, and T cells were significantly increased with co-exposure to OVA-ODE as compared to OVA alone. Lung ILC2 and ILC3 were only increased in OVA-Sal mice. Cytokine/chemokine levels varied with exposure to OVA-ODE reflecting an additive mixture of the pro- and allergic-inflammatory profiles. Collectively, ODE increased airway inflammatory cells and chemotactic mediator release in allergic (OVA) sensitized mice to suggest that persons with allergy/asthma be identified and warned prior to the occupational exposure of potentially worsening airway disease. [Description provided by NIOSH]
• Subjects:
  Agriculture Animals Asthma Asthma, Occupational Dust Immune System Lung Methacholine Compounds Occupational Health Respiratory System Safety

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• Keywords:
  Agricultural Industry Airway Disease Author Keywords: Agriculture Immune Reaction Inflammation Lung Tissue Methacholines Organic Dusts
• ISSN:
  1465-9921
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  51
• Volume:
  20
• NIOSHTIC Number:
  nn:20054993
• Citation:
  Respir Res 2019 Mar; 20:51
• Contact Point Address:
  Jill A. Poole, Pulmonary, Critical Care, Sleep & Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, 985900 Nebraska Medical Center, Omaha, NE 68198-5910
• Email:
  japoole@unmc.edu
• CAS Registry Number:
  Methacholine (CAS RN 55-92-5)
• Federal Fiscal Year:
  2019
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center - Omaha
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Respiratory Research
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:695afabe36826b49b36bb66736a9600f30f3fc7241edc74078aced0949e0ad52e5733bb74ff70a883a790bbd477c980bf9537bcacee9ece073a6228f21420d24
• Download URL:
  https://stacks.cdc.gov/view/cdc/217398/cdc_217398_DS1.pdf
• File Type:
  [PDF - 1.77 MB ]