A DNA Vaccine Expressing Consensus Hemagglutinin-Esterase Fusion Protein Protected Guinea Pigs from Infection by Two Lineages of Influenza D Virus

Details

• Personal Author:
  Daharsh L ; Fan W ; Kang G ; Li F ; Li Q ; Moriyama H ; Sreenivasan C ; Wan Y ; Wang D ; Zhang J
• Description:
  Two lineages of influenza D virus (IDV) have been found to infect cattle and promote bovine respiratory disease complex, one of the most commonly diagnosed causes of morbidity and mortality within the cattle industry. Furthermore, IDV can infect other economically important domestic livestock, including pigs, and has the potential to infect humans, which necessitates the need for an efficacious vaccine. In this study, we designed a DNA vaccine expressing consensus hemagglutinin-esterase fusion (HEF) protein (FluD-Vax) and tested its protective efficacy against two lineages of IDV (D/OK and D/660) in guinea pigs. Animals that received FluD-Vax (n = 12) developed appreciable titers of neutralizing antibodies against IDV lineage representatives, D/OK and D/660. Importantly, vaccinated animals were protected against intranasal challenge with IDV [3 x 10(5) 50% tissue culture infective dose(s) (TCID50)] D/OK (n = 6) or D/600 (n = 6), based on the absence of viral RNA in necropsied tissues (5 and 7 days postchallenge) using quantitative reverse transcription-PCR and in situ hybridization. In contrast, animals that received a sham DNA vaccine (n = 12) had no detectable neutralizing antibodies against IDV, and viral RNA was readily detectable in respiratory tract tissues after intranasal challenge [3 x 10(5) TCID50] with IDV D/OK (n = 6) or D/660 (n = 6). Using a TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay, we found that IDV D/OK and D/600 infections induced apoptosis in epithelial cells lining alveoli and bronchioles, as well as nonepithelial cells in lung tissues. Our results demonstrate for the first time that the consensus IDV HEF DNA vaccine can elicit complete protection against infection from two lineages of IDV in the guinea pig model. [Description provided by NIOSH]
• Subjects:
  Animal Husbandry Animals Antibodies Cattle Communicable Diseases Influenza, Human Livestock Occupational Health Respiratory System Respiratory Tract Diseases Safety Viral Diseases

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• Keywords:
  Animal-related Diseases Animal Husbandry Author Keywords: Influenza D Virus Cattle Industry Consensus Sequence DNA Vaccine Glycoproteins Guinea Pigs Hemagglutinin-esterase-fusion Protein Influenza Respiratory Diseases Vaccines Viral Diseases Viral Infections

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• ISSN:
  0022-538X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7 ; OSHA Region 8 ; South Dakota
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  92
• Issue:
  11
• NIOSHTIC Number:
  nn:20054848
• Citation:
  J Virology 2018 Jun; 92(11):e00110-18
• Contact Point Address:
  Qingsheng Li, Nebraska Center for Virology, University of Nebraska - Lincoln, Lincoln, Nebraska, USA
• Email:
  qli@unl.edu
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center - Omaha
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Journal of Virology
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:67aebba0f1abc186c0aa1c64e212ddcea93f8b0be71358799e97e712e55f07f7f3f35c33618cef47b2c05540c61798a0860962d8431f0917c77cda8559a32b8f
• Download URL:
  https://stacks.cdc.gov/view/cdc/217266/cdc_217266_DS1.pdf
• File Type:
  [PDF - 4.75 MB ]