Development of a Heterologous Enzyme-Linked Immunosorbent Assay for Organophosphorus Pesticides with Phage-Borne Peptide

Details

• Personal Author:
  Gee SJ ; Hammock BD ; Hua X ; Kim HJ ; Liu F ; Liu X ; Shi H ; Wang M ; Wang Y
• Description:
  An enzyme-linked immunosorbent assay (ELISA) was developed to detect organophosphorus pesticides using a phage-borne peptide that was isolated from a cyclic 8-residue peptide phage library. The IC50 values of the phage ELISA ranged from 1.4 to 92.1 ug L-1 for eight organophosphorus pesticides (parathion-methyl, parathion, fenitrothion, cyanophos, EPN, paraoxon-methyl, paraoxon, fenitrooxon). The sensitivity was improved 120- and 2-fold compared to conventional homologous and heterologous ELISA, respectively. The selectivity of the phage ELISA was evaluated by measuring its cross-reactivity with 23 organophosphorus pesticides, among which eight were the main cross-reactants. The spike recoveries were between 66.1% and 101.6% for the detection of single pesticide residues of parathion-methyl, parathion and fenitrothion in Chinese cabbage, apple and greengroceries, and all of the coefficients of variation were less than or equal to 15.9%. Moreover, the phage ELISA results were validated by gas chromatography. The results indicate that isolating phage-borne peptides from phage display libraries is an alternative method for the development of a heterologous immunoassay and that the developed assay has a lower limit of detection than the chemically synthesized competitor assay. [Description provided by NIOSH]
• Subjects:
  Enzymes Insecticides Occupational Health Organophosphates Safety
• Keywords:
  ELISA Enzyme-linked Immunosorbent Assay Enzyme Activity Immunoassays Organophosphorus Pesticides Pesticides And Agricultural Chemicals
• ISSN:
  2046-2069
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  4
• Issue:
  80
• NIOSHTIC Number:
  nn:20054012
• Citation:
  RSC Adv 2014 Aug; 4(80):42445-42453
• Contact Point Address:
  Minghua Wang, College of Plant Protection (State & Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China
• Email:
  wangmha@njau.edu.cn
• CAS Registry Number:
  Fenitrothion (CAS RN 122-14-5) ; Parathion (CAS RN 56-38-2)
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Royal Society of Chemistry Advances
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0a2bdf52ecc2023024343f4343e3fd4abe6656b39ebaad71c5ea2f6bddf9d55156b0da46cf3a206c42b30cfa3668f62a5dcfe3946325b7d86a2f3730b3739f9f
• Download URL:
  https://stacks.cdc.gov/view/cdc/216663/cdc_216663_DS1.pdf
• File Type:
  [PDF - 572.83 KB ]