A High Resolution/Accurate Mass (HRAM) Data-Dependent MS3 Neutral Loss Screening, Classification, and Relative Quantitation Methodology for Carbonyl Compounds in Saliva

Details

• Personal Author:
  Balbo S ; Carrà A ; Dator R ; Guidolin V ; Maertens L ; Villalta PW
• Description:
  Reactive carbonyl compounds (RCCs) are ubiquitous in the environment and are generated endogenously as a result of various physiological and pathological processes. These compounds can react with biological molecules inducing deleterious processes believed to be at the basis of their toxic effects. Several of these compounds are implicated in neurotoxic processes, aging disorders, and cancer. Therefore, a method characterizing exposures to these chemicals will provide insights into how they may influence overall health and contribute to disease pathogenesis. Here, we have developed a high resolution accurate mass (HRAM) screening strategy allowing simultaneous identification and relative quantitation of DNPH-derivatized carbonyls in human biological fluids. The screening strategy involves the diagnostic neutral loss of hydroxyl radical triggering MS3 fragmentation, which is only observed in positive ionization mode of DNPH-derivatized carbonyls. Unique fragmentation pathways were used to develop a classification scheme for characterizing known and unanticipated/unknown carbonyl compounds present in saliva. Furthermore, a relative quantitation strategy was implemented to assess variations in the levels of carbonyl compounds before and after exposure using deuterated d3-DNPH. This relative quantitation method was tested on human samples before and after exposure to specific amounts of alcohol. The nano-electrospray ionization (nano-ESI) in positive mode afforded excellent sensitivity with detection limits on-column in the high-attomole levels. To the best of our knowledge, this is the first report of a method using HRAM neutral loss screening of carbonyl compounds. In addition, the method allows simultaneous characterization and relative quantitation of DNPH-derivatized compounds using nano-ESI in positive mode. [Description provided by NIOSH]
• Subjects:
  Biomarkers Environmental Exposure Occupational Health Safety
• Keywords:
  Aldehydes/Carbonyl Compounds Author Keywords: High Resolution Accurate Mass Data-dependent MS3 Neutral Loss Screening Biological Effects Carbonyls Chemical Indicators DNPH-derivatization Environmental Exposure Exposome Relative Quantitation Screening Methods
• ISSN:
  1044-0305
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  28
• Issue:
  4
• NIOSHTIC Number:
  nn:20053769
• Citation:
  J Am Soc Mass Spectrom 2017 Apr; 28(4):608-618
• Contact Point Address:
  Silvia Balbo, Masonic Cancer Center, University of Minnesota, 2231 6th Street SE, Minneapolis, MN 55455, USA
• Email:
  balbo006@umn.edu
• Federal Fiscal Year:
  2017
• Performing Organization:
  University of Minnesota Twin Cities
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of the American Society for Mass Spectrometry
• End Date:
  20250630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ed2b1fb3be8b3c1d0a21941f2b2e9476b9c1932a65d9a69fbc274abfca26b8e00f17c6a1f9fb657ce298458a6535ab0f1eee77ff6b29c76cc7b1657a70675fc2
• Download URL:
  https://stacks.cdc.gov/view/cdc/216476/cdc_216476_DS1.pdf
• File Type:
  [PDF - 352.42 KB ]