High Throughput Genomic Sequencing of Bioaerosols in Broiler Chicken Production Facilities

Details

• Personal Author:
  Bair T ; Bray JL ; Chimenti MS ; Farnell M ; Nonnenmann MW ; O'Brien KM ; Tabler T ; Bair T ; Bray JL ; Chimenti MS ; Farnell M ; Nonnenmann MW ; O'Brien KM ; Tabler T
• Description:
  Chronic inhalation exposure to agricultural dust promotes the development of chronic respiratory diseases among poultry workers. Poultry dust is composed of dander, chicken feed, litter bedding and microbes. However, the microbial composition and abundance has not been fully elucidated. Genomic DNA was extracted from settled dust and personal inhalable dust collected while performing litter sampling or mortality collection tasks. DNA libraries were sequenced using a paired-end sequencing-by-synthesis approach on an Illumina HiSeq 2500. Sequencing data showed that poultry dust is predominantly composed of bacteria (64-67%) with a small quantity of avian, human and feed DNA (< 2% of total reads). Staphylococcus sp. AL1, Salinicoccus carnicancri and Lactobacillus crispatus were the most abundant bacterial species in personal exposure samples of inhalable dust. Settled dust had a moderate relative abundance of these species as well as Staphylococcus lentus and Lactobacillus salivarius. There was a statistical difference between the microbial composition of aerosolized and settled dust. Unlike settled dust composition, aerosolized dust composition had little variance between samples. These data provide an extensive analysis of the microbial composition and relative abundance in personal inhalable poultry dust and settled poultry dust. [Description provided by NIOSH]
• Subjects:
  Aerosols Agriculture Chronic Disease Dust Livestock Microbiology Occupational Health Respiratory System Safety
• Keywords:
  Agricultural Industry Chronic Diseases Dust Analysis Dust Inhalation Inhalation Studies Livestock Industry Microorganisms Organic Dusts
• ISSN:
  1751-7915
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  Iowa ; Mississippi ; OSHA Region 4 ; OSHA Region 6 ; OSHA Region 7 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  9
• Issue:
  6
• NIOSHTIC Number:
  nn:20053170
• Citation:
  Microb Biotechnol 2016 Nov; 9(6):782-791
• Contact Point Address:
  Matthew W. Nonnenmann, Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, USA
• Email:
  matthew-nonnenmann@uiowa.edu
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Agriculture, Forestry and Fishing ; Agriculture, Forestry and Fishing
• Performing Organization:
  University of Texas Health Center at Tyler
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Microbial Biotechnology
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3e5356ca4c314a40074c3b0e21290b48954db295abe1c50ae8f9b59507b46828069c722ec4628fb4cbc8d519b18cb0f9c0bf4464217108bd2b910b6b968439fa
• Download URL:
  https://stacks.cdc.gov/view/cdc/215982/cdc_215982_DS1.pdf
• File Type:
  [PDF - 573.41 KB ]