Culture-Independent Characterization of Bacteria in Poultry and Dairy Bioaerosols Using Pyrosequencing: A New Approach

Details

• Personal Author:
  Bextine B ; Dowd S ; Gilmore K ; Hussain A ; Levin J ; Nonnenmann M ; Ward W
• Description:
  Introduction: Culture-based methods are often used for characterization of bioaerosols. Limitations exist with culture-based methods as only microorganisms which are viable and able to grow on selected media can be characterized. A need exists to develop methodologies which are not subject to the limitations of culture-based characterization. Novel molecular techniques such as bacterial tagencoded flexible (FLX) amplicon pyrosequencing (bTEFAP) may be a useful for the characterizing of bioaerosols. Objective: Use bTEFAP to characterizing and estimate concentrations of bioaerosols in dairy and poultry facilities. Methods: bTEFAP was used to characterize inhalable bioaerosols present in poultry and dairy facilities over an eight-hour work shift. Both personal and area samples were collected using the IOM at 2 L/min and a gelatin filter. The DNA present was pyrosequenced targeting the 16S bacterial genetic region. This genetic region if often targeted for identifying bacteria in environmental microbiological studies. The relative percentages of bacteria present in each sample were reported. Results: Preliminary results suggest large distributions of bacteria among inhalable samples collected in poultry and dairy facilities. Of the bacteria detected, 369 genera were identified. The inhalable bacteria concentrations were estimated to be 7503 cells/m3 and 7657 cells/m3 in poultry and dairy facilities, respectively. Prevalent bacteria identified in the dairy facility: Papilibacter (83%), Clostridium (53%) and Clostridium lituseburense (51%). Bacteria identified in the poultry facility: Staphylococcus cohnii (23%), Staphylococcaceae (14%). Conclusions: Bioaerosols were characterized; however concentrations of bacteria were lower than previously reported and these bacteria may not be viable. This is the first application of pyrosequencing technology for the characterization of bioaerosols. Furthermore, the fast processing speed of molecular techniques may revolutionize the ability to identify the phylogeny and concentration of bioaerosols. The impact of this technology has yet to be realized by the scientific community dedicated to evaluating occupational and environmental bioaerosol exposure [Description provided by NIOSH]
• Subjects:
  Aerosols Agriculture Bacteria Dust Livestock Microbiology Occupational Health Safety
• Keywords:
  Agricultural Industry Bacterial Cultures Bacterial Dusts Dust Analysis Dust Inhalation Livestock Industry Microorganisms Organic Dusts
• Publisher:
  American Industrial Hygiene Association
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  81
• NIOSHTIC Number:
  nn:20053172
• Citation:
  AIHce 2011: American Industrial Hygiene Conference and Exposition, May 14-19, 2011, Portland, Oregon. Falls Church, VA: American Industrial Hygiene Association, 2011 May; :81
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Texas Health Center at Tyler
• Peer Reviewed:
  False
• Start Date:
  20010930
• Source Full Name:
  AIHce 2011: American Industrial Hygiene Conference and Exposition, May 14-19, 2011, Portland, Oregon
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1b26f144fdf50bc47da8a49580fcd2f758010e43bd84f64a8b3e48789f6a6090065bbf3df4b4ae0bf6074423a5051a3c7d99cd06a3a192b92a7a87e111a06e03
• Download URL:
  https://stacks.cdc.gov/view/cdc/215984/cdc_215984_DS1.pdf
• File Type:
  [PDF - 221.59 KB ]