Chronic Obstructive Pulmonary Disease Patients Have Greater Systemic Responsiveness to Ex Vivo Stimulation with Swine Dust Extract and Its Components Versus Healthy Volunteers

Details

• Personal Author:
  Gleason A ; Harting JR ; Poole JA ; Qiu F ; Romberger DJ ; Von Essen SG ; Gleason A ; Harting JR ; Poole JA ; Qiu F ; Romberger DJ ; Von Essen SG
• Description:
  Chronic obstructive pulmonary disease (COPD) is characterized by an airway and systemic inflammatory response. Bioaerosols/organic dusts are important agricultural pollutants that may lead to COPD. These environments are complex, containing a rich source of various microbial components. The objective of this study was to determine whether individuals with COPD have enhanced systemic responsiveness to settled swine facility organic dust extract (ODE) or its main pathogenic components (peptidoglycan [PGN], lipopolysaccharide [LPS]) versus healthy volunteers. A modified whole blood assay (WBA) that included occupational levels of ODE and concentrations of LPS and PGN found in ODE was used to determine systemic responsiveness (mediator release), and sputum inflammatory markers were measured to explore for systemic and airway associations. Sputum samples were evaluated for cell counts, and tumor necrosis factor (TNF)-a, interleukin (IL)-8/CXCL8, IL-6, and IL-10. Ex vivo whole blood stimulation with ODE, LPS, and PGN each resulted in significant mediator release in all subjects, with the highest occurring with ODE; PGN resulted in significantly enhanced TNF-a and IL-8 as compared to LPS. COPD subjects demonstrated greater systemic responsiveness using the modified WBA versus healthy controls. Within COPD subjects, blood baseline TNF-a, IL-8, and IL-10 and ODE-, PGN-, and LPS-stimulated IL-8 levels significantly correlated with lung function. In conclusion, dust-induced mediator release was robust, and PGN, in part, resembled dust-induced mediator release. Subjects with COPD demonstrated increased mediator release following ex vivo whole blood stimulation with bioaerosol components, suggesting that circulating blood cells in COPD subjects may be primed to respond greater to microbial/inflammatory insult. [Description provided by NIOSH]
• Subjects:
  Agriculture Dust Livestock Microbiology Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Agricultural Industry Bioaerosols Chronic Obstructive Pulmonary Disease COPD Dust Exposure Livestock Industry Microorganisms Organic Dusts Physiological Effects Physiological Response Respiratory Diseases Respiratory Function

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• ISSN:
  1528-7394
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  Nebraska ; North Carolina ; OSHA Region 4 ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  75
• Issue:
  24
• NIOSHTIC Number:
  nn:20053155
• Citation:
  J Toxicol Environ Health A 2012 Nov; 75(24):1456-1470
• Contact Point Address:
  Jill A. Poole, MD, Pulmonary, Critical Care, Sleep & Allergy Division, Department of Medicine, University of Nebraska Medical Center, 985300 The Nebraska Medical Center, Omaha, NE 68198-5300, USA
• Email:
  japoole@unmc.edu
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Agriculture, Forestry and Fishing ; Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a9063c724d3a0f1ecf31ab4b2229d2e54b3eb8c7063e015eefddf99fe88e63d3e9b5dc5301b778155fd0e8f56302035c0277fd3a6c2d76f40fcdc26c8d8ce11f
• Download URL:
  https://stacks.cdc.gov/view/cdc/215969/cdc_215969_DS1.pdf
• File Type:
  [PDF - 179.78 KB ]