Modulation of Fct Receptor Expression and Function in Mouse Peritoneal Macrophages by Ammonium Metavanadate

Details

• Personal Author:
  Vaddi K ; Wei C-I
• Description:
  Resident peritoneal macrophages (PEM) harvested from female B6C3F1 mice given an intraperitoneal injection of ammonium metavanadate (2.5 or 10 mg V/kg), an equivalent amount of ammonium in the form of ammonium chloride, or sodium phosphate buffer (0.1 M, pH 7.2) every third day for 6 weeks, were subjected to flow cytometric analysis of Fct2a and Fct2b receptor expression, and photometric microassay to measure receptor mediated binding and phagocytosis of sheep red blood cells (SRBC). The NH4Cl and 10V groups showed 21.7 and 17.2% lower mean fluorescence channel (MFC) values and 7.1 and 5.9% lower values in percentage fluorescence-positive cells than the phosphate buffer control with respect to Fct2a expression. For Fct2b expression, the 10V group showed significantly (P<0.05) lower MFC (31.2%) and percentage fluorescence-positive cells (15.7%) than the phosphate buffer control. Though the four groups did not show a significant difference in Fct2a mediated binding and phagocytosis of SRBC, the 10V group showed a significantly lower Fct2b mediated binding and phagocytosis. The results indicate that the reduction in Fct2b expression and function could contribute toward the previously observed depression in phagocytosis, NADPH-oxidase and superoxide generation in peritoneal macrophages obtained from vanadate-treated animals. [Description provided by NIOSH]
• Subjects:
  Animals Occupational Health Safety Vanadium
• Keywords:
  Ammonium Compounds Animal Studies Cellular Effects Phagocytes Phagocytic Activity Vanadium Compounds Vanadium Oxides
• ISSN:
  0192-0561
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Florida ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  13
• Issue:
  8
• NIOSHTIC Number:
  nn:20058025
• Citation:
  Int J Immunopharmacol 1991 Aug; 13(8):1167-1176
• Contact Point Address:
  Cheng-I Wei, Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611-0163 U.S.A.
• CAS Registry Number:
  Sodium vanadium oxide (Na3VO4) (CAS RN 13721-39-6)
• Federal Fiscal Year:
  1991
• Performing Organization:
  University of Florida, Gainesville, Florida
• Peer Reviewed:
  True
• Start Date:
  19880401
• Source Full Name:
  International Journal of Immunopharmacology
• End Date:
  19910331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1187ca5ede0be55a0e8c7949ada7cfb5a2850d3c643ef24cf42ddabf7051344282fb95b4b7f2e72cce23fa100f5bca40c1b651c3abab29e17ad86ee4be99b878
• Download URL:
  https://stacks.cdc.gov/view/cdc/215216/cdc_215216_DS1.pdf
• File Type:
  [PDF - 703.13 KB ]