Binding of Anti-Acetaldehyde IgG Antibodies to Hepatocytes with an Acetaldehyde-Phosphatidylethanolamine Adduct on Their Surface

Details

• Personal Author:
  Allen K ; Ardies CM ; Green CE ; Trudell JR
• Description:
  We have previously shown that antibodies raised against acetaldehyde adducts of protein cross-react with an acetaldehyde adduct of dioleoylphosphatidylethanolamine, N-ethyl-dioleoylphosphatidylethanolamine, when the latter is incorporated into hexagonal phase phospholipid micelles. In the present study we demonstrate that these same IgG antibodies cross-react with N-ethyl-dioleoylphosphatidylethanolamine when this adduct is incorporated into the surface of hepatocytes. Hapten-specific IgG antibodies were purified from the sera of rabbits sensitized to an albumin-acetaldehyde conjugate that had been reduced with sodium cyanoborohydride (N-ethy-RSA). The N-ethyl-RSA was coupled to an Affi-Gel-10 column to affinity purify the lgG. Liposomes containing N-ethyl-dioleoylphosphatidylethanolamine were fused with isolated hepatocytes, the affinity purified primary IgG antibodies were added, then fluoresceinconjugated second antibodies were added, and antibody binding to hepatocytes was measured by flow cytometry. The fluorescence of these hepatocytes was significantly greater (p < 0.01) than control hepatocytes prepared with (1) pre-immune primary IgG antibodies with fluorescein-conjugated second antibodies, (2) no primary antibody but with fluorescein-conjugated second antibodies, and (3) no fluorescein-conjugated second antibodies. [Description provided by NIOSH]
• Subjects:
  Alcoholic Beverages Antibodies Immune System Lipids Occupational Health Safety
• Keywords:
  Acetaldehyde Alcohol Antibody Response Author Keywords: Alcohol Hepatocytes Immune Reaction Phospholipid Phospholipids
• ISSN:
  0145-6008
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; Illinois ; OSHA Region 5 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  295-299
• Volume:
  15
• Issue:
  2
• NIOSHTIC Number:
  nn:20057967
• Citation:
  Alcohol Clin Exp Res 1991 Mar/Apr; 15(2):295-299
• Contact Point Address:
  J. R. Trudell, PhD, Department of Anesthesia, Stanford University School of Medicine, Stanford, CA 94305-5123
• Federal Fiscal Year:
  1991
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Alcoholism: Clinical and Experimental Research
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:568bba7b8486cc8a31d1edb42834ffb053d5be8d4a562341e38d5012a814f9f34ce0557d8cb689c5e014eb13bfd1200c9784f5810787c22032ff663165f7107e
• Download URL:
  https://stacks.cdc.gov/view/cdc/215166/cdc_215166_DS1.pdf
• File Type:
  [PDF - 493.45 KB ]