Modified Method of Mammalian Cell Synchronization Improves Yield and Degree of Synchronization

Details

• Personal Author:
  Cao G ; Cleary SF ; Liu L-M
• Description:
  A modified method to synchronize CHO and HeLa cells is developed based upon a combined shaking-off and chemical blockage. This method has effectively blocked quiescient cells, which is the main obstacle of high degree synchronization. Flowcytometry data show the improvement on the degree of synchronization and yield compared to two previously used methods. [Description provided by NIOSH]
• Subjects:
  Enzymes Laboratories Occupational Health Safety
• Keywords:
  Cell Biology Cellular Function Electromagnetic Radiation Enzyme Activity Laboratory Techniques Radiation Effects Radiation Exposure
• ISSN:
  0014-4827
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  405-410
• Volume:
  193
• Issue:
  2
• NIOSHTIC Number:
  nn:20057966
• Citation:
  Exp Cell Res 1991 Apr; 193(2):405-410
• Contact Point Address:
  Stephen F. Cleary, Department of Physiology and Biophysics, Box 551, MCV Station, Virginia Commonwealth University, Richmond, VA 23298-0551
• Federal Fiscal Year:
  1991
• Performing Organization:
  Virginia Commonwealth University, Richmond, Virginia
• Peer Reviewed:
  True
• Start Date:
  19850927
• Source Full Name:
  Experimental Cell Research
• End Date:
  19930929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e59777feb8bcd1a57fab336f779cea94dc4496c6f6ba0dd2a112f58d4a6be3b65cbdad3c72f380317b1e000b0ff77c7689c9e748a73c0c9d97a9b26fc3964e6c
• Download URL:
  https://stacks.cdc.gov/view/cdc/215165/cdc_215165_DS1.pdf
• File Type:
  [PDF - 467.32 KB ]