Immunologic Measurement of Polycyclic Aromatic Hydrocarbon-Albumin Adducts in Foundry Workers and Roofers

Details

• Personal Author:
  Hemminki K ; Herbert R ; Lee BM ; Perera FP ; Santella RM ; Yin BY
• Description:
  Roofers and iron foundry workers with high exposure to polycyclic aromatic hydrocarbons (PAH) were monitored for levels of covalent PAH serum albumin adducts, quantitated in enzymatically digested samples by a sensitive competitive enzyme-linked immunosorbent assay. Albumin adducts were higher in the foundry workers (5.22 fmol/micrograms, 0.314 mmol/mol) than in the reference group (4.07 fmol/micrograms, 0.245 mmol/mol), but only of borderline significance probably due to the small sample size. In a subset of foundry workers, a significant difference in adduct levels was observed for samples collected immediately after vacation and after six weeks of workplace exposure. The roofers also showed higher levels of adducts (5.19 fmol/micrograms, 0.312 mmol/mol) than their reference group (3.28 fmol/micrograms, 0.197 mmol/mol). These results demonstrate the feasibility of PAH protein adduct measurement as a marker of human exposure to this class of chemicals. [Description provided by NIOSH]
• Subjects:
  Biomarkers Construction Industry Enzymes Occupational Health Safety
• Keywords:
  Author Keywords: Albumin Adducts Benzo[a]pyrene ELISA Enzyme-linked Immunosorbent Assay Foundry Workers Immunological Tests Monoclonal Antibodies PAH Polynuclear Aromatic Hydrocarbons Roofers
• ISSN:
  0355-3140
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  190-194
• Volume:
  17
• Issue:
  3
• NIOSHTIC Number:
  nn:20057965
• Citation:
  Scand J Work Environ Health 1991 Jun; 17(3):190-194
• Contact Point Address:
  Dr RM Santella, Columbia University, Division of Environmental Sciences, 650 W 168th Street, New York, NY 10032, USA
• Federal Fiscal Year:
  1991
• Performing Organization:
  Columbia University, New York
• Peer Reviewed:
  True
• Start Date:
  19890101
• Source Full Name:
  Scandinavian Journal of Work, Environment & Health
• End Date:
  19921231
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:25ba37a85516519faeb31ea7d39d065bb6ca4f2dd80133fcab528dac7e977458d6658e94cfc2edbba62ad40b4048e2e3257b4d90e1af4ba6d2501722ad672575
• Download URL:
  https://stacks.cdc.gov/view/cdc/215164/cdc_215164_DS1.pdf
• File Type:
  [PDF - 3.17 MB ]