Regulation of the Growth of Mouse Leydig Cells by the Inactive Stereoisomer, 17alpha-Estradiol: Lack of Correlation Between the Elevated Expression of ERalpha and Difference in Sensitivity to Estradiol Isomers

Details

• Personal Author:
  DuMond JW Jr. ; Roy D ; Singh KP
• Description:
  We examined the effects of 17a-estradiol, the biologically inactive stereoisomer of 17B-estradiol, on cell growth and cell cycle kinetics using the normalized mouse TM3 Leydig cells. A significant biphasic stimulatory growth response was observed by 17a-estradiol exposure with peaks at 1 pg/ml (157.13%) and 100 ng/ml (120.04%) (p<0.05). The growth stimulatory effects of 17a-estradiol were inhibited by tamoxifen. A significant decrease in cell cycle time of Leydig cells exposed to 17a-estradiol was observed in treated cells (p<0.05). RT-PCR analysis indicated that exposure to Leydig cells to 1 pg/ml and 100 ng/ml 17a-estradiol resulted in a 10- and 5-fold increases in the expression of ERa, respectively. Similar effects were observed with exposure to equivalent concentrations of 17B-estradiol. Difference in sensitivity to stereoisomers of estradiol to growth response of Leydig cells did not correlate with the elevated expression of ERa. We conclude that the TM3 Leydig cells are sensitive to the non-typical estrogen, 17a-estradiol, presumably through the activation of ER-independent signaling transduction pathways. [Description provided by NIOSH]
• Subjects:
  Animals Estrogens Occupational Health Safety Urogenital System
• Keywords:
  Animal Studies Author Keywords: Cell Cycle Cell Signaling Cellular Effects ERa ERB Estrogen Estrogenic Hormones Gene Expression Leydig Cells Testes

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• ISSN:
  1021-335X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Alabama ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  8
• Issue:
  4
• NIOSHTIC Number:
  nn:20057620
• Citation:
  Oncol Rep 2001 Jul; 8(4):899-902
• Contact Point Address:
  Dr Deodutta Roy, Department of Environmental Health Sciences, The University of Alabama at Birmingham, 1665 University Blvd., 317 Ryals Building, Birmingham, AL 35294- 0022, USA
• Email:
  Royd@uab.edu
• Federal Fiscal Year:
  2001
• Performing Organization:
  Deep South Center for Occupational Health and Safety, University of Alabama at Birmingham, Birmingham, AL
• Peer Reviewed:
  True
• Start Date:
  19980701
• Source Full Name:
  Oncology Reports
• End Date:
  20040630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2935b603a07a8833db941fdee501e7fae9150766a020f8b31ab19d71973f004c754825a31be2ff64acf20a362a908f65cd68325a25250a950f41cf6c768a5270
• Download URL:
  https://stacks.cdc.gov/view/cdc/214893/cdc_214893_DS1.pdf
• File Type:
  [PDF - 969.72 KB ]