Immune Phenotype and Interleukin-6 Deficiency Influence the Cytokine Profile and Inflammatory Cell Presence in Murine Skin in Response to Irritant Contact Dermatitis

Details

• Personal Author:
  Calhoun K ; Frempah B ; Gallucci R ; Luckett-Chastain L ; Schartz T
• Description:
  Irritant contact dermatitis (ICD) is an acute inflammatory response induced by exposure to topical irritants, and is labeled as the most common occupational skin disease by the Centers for Disease Control and Prevention. Currently, identifying potential therapeutic targets and predicting an individual's response to irritant exposure is hindered by the interpatient variability associated with the manifestation and severity of ICD. However, immune phenotype, such as Th1- and Th2-domiance, is postulated to contribute to this variability. In addition, interleukin 6 (IL-6), which displays both Th1 and Th2 properties, has previously been shown to play a role in the severity of ICD. C57BL/6J ("Th1 dominant"), Balb/c ("Th2 dominant"), and IL-6KO mice were utilized to investigate ICD induced by exposure to the occupational irritants, benzoalkonium chloride (BKC) and JP-8 jet fuel. Histopathology revealed epidermal thickening and dermal cellular infiltration in dermatitis lesions, with IL- 6KO skin exhibiting the most severe damage. Analysis of skin cytokine and chemokine protein expression showed elevated levels of IL-12 and IL-15 in C57 mice exposed to irritants as compared to Balb/c mice. Interestingly, flow cytometric analysis also revealed greater infiltration of CD11b+, Ly6G+ cells in dermatitis lesions from C57 mice. However, Balb/c mice expressed more Th2-associated cytokines following irritant exposure than C57 mice. In response to BKC, IL-6KO mice exhibited elevated IL-1a expression compared to C57 mice. Also, JP-8 exposure in IL-6KO mice resulted in down-regulation of IL-18 and TGF- B as well as decreased CD11b+, Ly6G+ cellular presence in comparison to C57 mice. Overall, it appears that immune phenotype and IL-6 deficiency alter the skin inflammatory response in ICD via differential cytokine and chemokine expression which influences the type of inflammatory cells that infiltrate into the skin. These findings provide a better understanding of the pathophysiology of ICD and insight into potential targets for therapeutic immunomodulation. [Description provided by NIOSH]
• Subjects:
  Animals Immune System Irritants Occupational Health Safety Skin
• Keywords:
  Animal Studies Contact Dermatitis Cytokines Genetic Factors Immune Reaction Skin Exposure
• Publisher:
  University of Oklahoma Health Sciences Center
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Oklahoma ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  65
• NIOSHTIC Number:
  nn:20057011
• Citation:
  41st Annual Symposium: Graduate Research Education and Technology (GREAT), March 28-31, 2016, Oklahoma City, Oklahoma. Oklahoma City, OK; University of Oklahoma Health Sciences Center, 2016 Mar; :65
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing ; Oil and Gas Extraction
• Performing Organization:
  University of Oklahoma Health Sciences Center, Oklahoma City
• Peer Reviewed:
  False
• Start Date:
  20130901
• Source Full Name:
  41st Annual Symposium: Graduate Research Education and Technology (GREAT), March 28-31, 2016, Oklahoma City, Oklahoma
• End Date:
  20180831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cec0df5bda6214d75dda84957b432fbb9f7926770c5672567683280bf5d5bc73bdb290c4e9046b8dffe2b6a25e954730e7fff52e19d6042c3d95ab8d352b6b44
• Download URL:
  https://stacks.cdc.gov/view/cdc/214494/cdc_214494_DS1.pdf
• File Type:
  [PDF - 111.38 KB ]