Immune Phenotype and IL-6 Deficiency Modulate the Inflammatory Response in a Mouse Model of Irritant Contact Dermatitis

Details

• Personal Author:
  Calhoun K ; Gallucci R ; Luckett-Chastain L
• Description:
  Skin diseases are the second most common occupational illness with associated per annum costs exceeding $1 billion. Irritant contact dermatitis (ICD), the most common occupational skin disease, is characterized as an acute inflammatory response that manifests as a result of topical irritant exposure. Immune phenotype, such as Th1- and Th2- dominance, is postulated to contribute to the variability seen between patients. In addition, interleukin 6, which is known to have variably both Th1 and Th2 properties, has been shown to play a key role in ICD. C57BL/6J ("Th1 dominant"), Balb/c ("Th2 dominant"), and IL-6KO mice were utilized to investigate ICD induced by exposure to the occupational irritants, benzoalkonium chloride (BKC) and JP-8 jet fuel. Histopathology revealed epidermal thickening and dermal cellular infiltration in dermatitis lesions, with IL-6KO skin exhibiting the most severe damage. Analysis of skin cytokine and chemokine protein expression showed that IL-1β, TNF-a, TGF-β, CCL2, CCL3 and CXCL1, were elevated in mice exposed to BKC regardless of immune phenotype. In contrast, IL-1β, TNF-a, TGF-β, and CXCL1 varied between mouse strains when JP-8 was compared to control exposure. Flow cytometric analysis and immunohistochemistry indicated neutrophil and monocyte presence within dermatitis lesions; however, IL-6KO showed a greater amount of neutrophils following BKC exposure and decreased neutrophil presence after JP-8 exposure when compared to C57 mice. Overall, it appears that immune phenotype and IL-6 deficiency alters the skin inflammatory response in ICD via differential cytokine and chemokine expression that influence the type of inflammatory cells that infiltrate into the skin. [Description provided by NIOSH]
• Subjects:
  Animals Immune System Irritants Occupational Health Safety Skin
• Keywords:
  Animal Studies Contact Dermatitis Cytokines Genetic Factors Immune Reaction Skin Exposure
• ISSN:
  0022-1767
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Oklahoma ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  196
• Issue:
  1
• NIOSHTIC Number:
  nn:20057004
• Citation:
  J Immunol 2016 May; 196(1)(Suppl):126.34
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing ; Oil and Gas Extraction
• Performing Organization:
  University of Oklahoma Health Sciences Center, Oklahoma City
• Peer Reviewed:
  False
• Start Date:
  20130901
• Source Full Name:
  The Journal of Immunology
• Supplement:
  Supplement
• End Date:
  20180831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2e067a56233c2c603d474207334a3130e5e3ceed6e5b0c3ec30d85d48c0a28ed296650ea4794c6d795d3950a9eacbda82467c172919384c7e1c7be0563767a24
• Download URL:
  https://stacks.cdc.gov/view/cdc/214487/cdc_214487_DS1.pdf
• File Type:
  [PDF - 84.33 KB ]