Dermatological Effects of Weathered Petroleum Exposure

Details

• Personal Author:
  Gallucci R ; Kemp J ; Luckett-Chastain L ; McShan W
• Description:
  Nearly 5 million barrels of oil were released into the Gulf of Mexico following the Deepwater Horizon oil well failure, making it undoubtedly the largest environmental disaster response effort in U.S. history. Spill remediation workers were continuously exposed to weathered oil and appeared to suffer a myriad of health effects, including contact dermatitis. However, the mechanism by which crude oil exposure induces skin inflammation has not been examined. To investigate this pathology, a mouse model of seven day exposure to weathered crude oil or acetone (control) was utilized, employing C57BL/6 and Balb/c mice. Immunohistochemistry, multiplex protein analysis, and real-time PCR were then conducted on skin samples isolated from each strain. Inflammatory cytokine expression showed that oil exposure increased IL-6, CXCL10, and CCL2 levels, the extent of which differed based on mouse strain. Histopathology showed obvious inflammation in the oil exposed skin, primarily characterized by epidermal thickening and neutrophil infiltration, and this was most pronounced in Balb/c mice. Oil exposure increased M1 macrophage skin infiltration but not M2 as indicated by CD86 versus CD206 expression respectively. These results suggest that weathered oil is indeed a skin irritant, and genetic predisposition may influence the severity of the skin inflammation. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Pollution Immune System Occupational Health Petroleum Safety Skin
• Keywords:
  Animal Studies Contact Dermatitis Crude Oil Environmental Contamination Immune Reaction Skin Exposure
• ISSN:
  0892-6638
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Oklahoma ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  28
• NIOSHTIC Number:
  nn:20057009
• Citation:
  FASEB J 2014 Apr; 28(Suppl 1):844.18
• CAS Registry Number:
  Petroleum (CAS RN 8002-05-9)
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing ; Oil and Gas Extraction
• Performing Organization:
  University of Oklahoma Health Sciences Center, Oklahoma City
• Peer Reviewed:
  False
• Start Date:
  20130901
• Source Full Name:
  The FASEB Journal
• Supplement:
  1
• End Date:
  20180831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7557b645238e36d6fdae39ce68c02ada0edee54df3a5080394852b0e21f569971721d762523c5b5cbf7ebff98f591185aee5eb6f590da8e18a7d603ac3a0ba66
• Download URL:
  https://stacks.cdc.gov/view/cdc/214492/cdc_214492_DS1.pdf
• File Type:
  [PDF - 58.85 KB ]