IL-6 Deficiency Leads to a Specific Cytokine Profile During Irritant Contact Dermatitis

Details

• Personal Author:
  Calhoun KN ; Gallucci R ; Kemp JM ; Luckett-Chastain LR
• Description:
  Of reported occupational injury associated with workman's compensation, contact dermatitis ranks second most prevalent over all. Irritant contact dermatitis (ICD) is an acute inflammatory response that can vary between individuals. This difference between patients may be associated with varied modulation of the expression of specific inflammatory cytokines as a result of genetic or epigenetic alterations. This laboratory recently reported that mice deficient in the inflammatory cytokine interleukin 6 (IL-6KO) display worse ICD compared to WT in response to various chemical irritants. To further investigate this finding, denuded skin of IL-6KO and WT C57BL/6 mice was exposed to an irritant (benzalkonium chloride, BKC) or acetone (control) daily for 7 days. Histopathology revealed worse inflammation in the BKC exposed IL-6KO skin, primarily characterized by epidermal thickening and inflammatory cell infiltration. Multiplex protein analysis of skin inflammatory cytokine expression showed certain cytokines such as TNFa, IL-1B, CXCL2 were equally induced by irritancy in either mouse strain. However, G-CSF (2.5 fold), and IFNy (4.3 fold) were significantly increased in IL-6KO skin, while other cytokines showed decreases, such as IL-12 (-8.4 fold), IL-18 (-2.4 fold), IL-22 (-2.1 fold), CXCL10 (-3.4 fold), CCL4 (-2.1 fold), CCL5 (-3.5 fold), and CCL7 (-2.1 fold). Cytokine mRNA expression also varied; however, it did not necessarily correspond with protein expression. Flow cytometric and mRNA analysis of the macrophage markers F4/80 and CD206 indicated that M1 macrophages dominate the response in IL-6KO skin. Thus, it appears that IL-6 deficiency exacerbates skin irritation, and characteristic inflammatory cytokine expression and possibly macrophage phenotypic changes may drive the altered response. [Description provided by NIOSH]
• Subjects:
  Animals Immune System Irritants Occupational Health Safety Skin
• Keywords:
  Animal Studies Contact Dermatitis Cytokines Immune Reaction Skin Exposure
• ISSN:
  1096-6080
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; Oklahoma ; OSHA Region 6 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  144
• Issue:
  1
• NIOSHTIC Number:
  nn:20057008
• Citation:
  Toxicologist 2015 Mar; 144(1):519
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing ; Oil and Gas Extraction
• Performing Organization:
  University of Oklahoma Health Sciences Center, Oklahoma City
• Peer Reviewed:
  False
• Start Date:
  20130901
• Source Full Name:
  The Toxicologist. Society of Toxicology 54th Annual Meeting and ToxExpo, March 22-26, 2015, San Diego, California
• End Date:
  20180831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9047b06841d58dae83a17a89e92b00356251bc9e788ca511a9f32ac1941455a58683feeee6c4a51126ac416bbbcf40bf6609786d76280b47b4c0af62658edf2a
• Download URL:
  https://stacks.cdc.gov/view/cdc/214491/cdc_214491_DS1.pdf
• File Type:
  [PDF - 228.28 KB ]