Immunological Difference in Th1 and Th2 Dominant Mouse Strains in an ICD Model

Details

• Personal Author:
  Calhoun K ; Gallucci R ; Kemp J ; Luckett-Chastain L
• Description:
  Of reported occupational injury associated with workman's compensation, contact dermatitis ranks second most prevalent overall. C57BL/6 and BALB/c differ in their immune responses, tending to be either Th1- or Th2-dominant, respectively. Irritant contact dermatitis (ICD) is an acute inflammatory response that can vary among individuals. Therefore; to better characterize ICD, these two mouse strains were utilized. C57 and BALB/c mice were exposed to benzoalkonium chloride, (BKC) or acetone (control) for 3 or 7 days. Multiplex protein analysis showed pro-inflammatory cytokines such as IL-1β, IL-6, TNF-a, IFN-y, IL-22, and CSFs (GM-CSF and G-CSF) were all significantly upregulated in C57s at Day 3 as compared to BALB/c. Inflammatory cell chemoattractants such as CXCL1, 2, and 10, as well as CCL3, 4, 5, and 7 also displayed higher expression in C57s. Interestingly, it wasn't until Day 7 that BALB/c mice showed significantly elevated levels of inflammatory mediators such as IL-2, IL-4, IL-5, IL-31, IL-9, IFN-y, and CCL2 compared to C57. Cytokine mRNA expression also varied between the two strains, but did not necessarily correlate with protein data. In addition, C57 appeared to have worse inflammation than BALB/c as indicated via histopathology. Overall, these results further confirm previous findings in which BALB/c and C57 exhibit different immune responses and may provide a better understanding of dermopathology and allow for specific immunomodulation for therapy. [Description provided by NIOSH]
• Subjects:
  Animals Immune System Irritants Occupational Health Safety Skin
• Keywords:
  Animal Studies Contact Dermatitis Cytokines Immune Reaction Skin Exposure
• ISSN:
  0022-1767
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Oklahoma ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  194
• Issue:
  1
• NIOSHTIC Number:
  nn:20057007
• Citation:
  J Immunol 2015 May; 194(1)(Suppl):199.13
• CAS Registry Number:
  Petroleum (CAS RN 8002-05-9)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing ; Oil and Gas Extraction
• Performing Organization:
  University of Oklahoma Health Sciences Center, Oklahoma City
• Peer Reviewed:
  False
• Start Date:
  20130901
• Source Full Name:
  The Journal of Immunology
• Supplement:
  Supplement
• End Date:
  20180831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:44fc4173e2a1b51eb6cb10cd62763e1751afc576121f6b9f175ed43a738bd28713fe0d50559f33bae0828db2b43bf5eac230f13cde18a12f4ce66d58cfbd54f5
• Download URL:
  https://stacks.cdc.gov/view/cdc/214490/cdc_214490_DS1.pdf
• File Type:
  [PDF - 81.17 KB ]