Lack of an Association Between Environmental Exposure to Polychlorinated Biphenyls and P, P’-DDE and DNA Damage in Human Sperm Measured Using the Neutral Comet Assay

Details

• Personal Author:
  Altshul L ; Chen Z ; Hauser R ; Singh NP
• Description:
  BACKGROUND: Chlorinated organic chemicals, such as polychlorinated biphenyls (PCB), hexachlorobenzene (HCB), dichlorodiphenyl trichloroethane (DDT), and dichlorodiphenyl dichloroethene (DDE, the most stable daughter compound of DDT) are persistent lipophilic compounds found in a large portion of the general population. To explore the hypothesis that environmental exposure to these compounds is associated with altered DNA integrity in human sperm, a study of 212 male partners of a sub-fertile couple who presented to the Massachusetts General Hospital Andrology Laboratory was conducted. METHODS: The neutral single cell microgel electrophoresis assay (comet assay) was used to assess DNA integrity in sperm. VisComet image analysis software was used to measure total comet length, the proportion of DNA present in the comet tail, and tail distributed moment, an integrated measure of length and intensity. RESULTS: In the regression analyses, there were no statistically signifcant consistent associations between the comet assay parameters and any of the individual PCB congeners, sum of PCB, or p,p'-DDE. CONCLUSION: These results suggest that there are not strong relationships between adult levels of these chlorinated organic compounds and sperm DNA damage as measured by the comet assay. [Description provided by NIOSH]
• Subjects:
  Environmental Exposure Men Occupational Health Polychlorinated Biphenyls Safety Urogenital System
• Keywords:
  Author Keywords: Comet Assay DDT Deoxyribonucleic Acids Dichlorodiphenyltrichloroethane DNA DNA Damage Environmental Exposure Fertility Hexachloro Compounds Humans Organic Chemicals Organic Compounds PCBs Polychlorinated Biphenyls Reproduction Reproductive Effects Sperm

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• ISSN:
  0268-1161
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  18
• Issue:
  12
• NIOSHTIC Number:
  nn:20056538
• Citation:
  Hum Reprod 2003 Dec; 18(12):2525-2533
• Contact Point Address:
  R. Hauser, Occupational Health Program, Harvard School of Public Health, Building 1, Room 1405, 665 Huntington Ave., Boston, MA 02115
• Email:
  rhauser@hohp.harvard.edu
• CAS Registry Number:
  1,1′-Biphenyl, chloro derivs. (CAS RN 1336-36-3) ; DDT (CAS RN 50-29-3) ; Hexachlorobenzene (CAS RN 118-74-1) ; p,p′-DDE (CAS RN 72-55-9)
• Federal Fiscal Year:
  2004
• Performing Organization:
  Harvard School of Public Health
• Peer Reviewed:
  True
• Start Date:
  20030731
• Source Full Name:
  Human Reproduction
• End Date:
  20050630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a2975bbd64d4a496c15e6e1b0f8cd486e483d5188bb4cd3e33be89faa9895f40b4ad96c214fbfa59fd49921d2c74a00cb5e569fce3232010fb9752f597c14b7b
• Download URL:
  https://stacks.cdc.gov/view/cdc/214129/cdc_214129_DS1.pdf
• File Type:
  [PDF - 137.73 KB ]