Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011

Nguyen, Ha T.; Nguyen, Tung; Mishin, Vasiliy P.; Sleeman, Katrina; Balish, Amanda; Jones, Joyce; Creanga, Adrian; Marjuki, Henju; Uyeki, Timothy M.; Nguyen, Dang H.; Nguyen, Diep T.; Do, Hoa T.; Klimov, Alexander I.; Davis, Charles T.; Gubareva, Larisa V.

doi:10.3201/eid1912.130705

i

Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011

Supporting Files Public Domain

Dec 2013
By Nguyen, Ha T. ; Nguyen, Tung ; Mishin, Vasiliy P. ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Nguyen, Ha T. ; Nguyen, Tung ; Mishin, Vasiliy P. ; Sleeman, Katrina ; Balish, Amanda ; Jones, Joyce ; Creanga, Adrian ; Marjuki, Henju ; Uyeki, Timothy M. ; Nguyen, Dang H. ; Nguyen, Diep T. ; Do, Hoa T. ; Klimov, Alexander I. ; Davis, Charles T. ; Gubareva, Larisa V.
Description:

We assessed drug susceptibilities of 125 avian influenza A(H5N1) viruses isolated from poultry in Vietnam during 2009-2011. Of 25 clade 1.1 viruses, all possessed a marker of resistance to M2 blockers amantadine and rimantadine; 24 were inhibited by neuraminidase inhibitors. One clade 1.1 virus contained the R430W neuraminidase gene and reduced inhibition by oseltamivir, zanamivir, and laninamivir 12-, 73-, and 29-fold, respectively. Three of 30 clade 2.3.4 viruses contained a I223T mutation and showed 7-fold reduced inhibition by oseltamivir. One of 70 clade 2.3.2.1 viruses had the H275Y marker of oseltamivir resistance and exhibited highly reduced inhibition by oseltamivir and peramivir; antiviral agents DAS181 and favipiravir inhibited H275Y mutant virus replication in MDCK-SIAT1 cells. Replicative fitness of the H275Y mutant virus was comparable to that of wildtype virus. These findings highlight the role of drug susceptibility monitoring of H5N1 subtype viruses circulating among birds to inform antiviral stockpiling decisions for pandemic preparedness.
Subjects:

Animals Antiviral Agents Cell Line Drug Resistance, Viral Ferrets Hemagglutinin Glycoproteins, Influenza Virus Influenza A Virus, H5N1 Subtype Influenza In Birds Inhibitory Concentration 50 Microbial Sensitivity Tests Mutation Neuraminidase Phylogeny Poultry Public Health Surveillance Research Virus Replication
Source:

Emerg Infect Dis. 19(12):1963-1971.
Pubmed ID:

24274711
Pubmed Central ID:

PMC3840871
Document Type:

Journal Article
Place as Subject:

Vietnam
Location:

Asia
Volume:

19
Issue:

12
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:50ad4e711cd8dc7f60e0ae43ae84ff1b163c5c3237b5125df20e576a830054ce
Download URL:

https://stacks.cdc.gov/view/cdc/21361/cdc_21361_DS1.pdf
File Type:

[PDF - 635.07 KB ]

File Language:

English

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