Occupational exposure of dairy farm workers and working environment to multidrug resistant Salmonella enterica.

Details

• Personal Author:
  Daniels J ; Magnuson R ; Murray K ; Nealon NJ ; Palomares Velosa JE ; Rao S ; Román-Muñiz IN ; Scaria J ; Daniels J ; Magnuson R ; Murray K ; Nealon NJ ; Palomares Velosa JE ; Rao S ; Román-Muñiz IN ; Scaria J
• Description:
  Objective: Dairy cattle operations are a working environment with a high risk for occupational exposure to antimicrobial resistant (AMR) Salmonella enterica among workers. Despite the risk, there is a lack of research on their spread in dairy farms. The objectives of our study were to characterize AMR of S. enterica serovar Dublin from worker clothing and environment and perform genetic profiling. Our hypothesis was that S. Dublin from farm worker clothing and environment would identify diverse AMR patterns and genes revealing potential exposure of workers to health hazards. Methods: Dairy farm workers were recruited for participation from two Colorado farms, swab samples from clothing (bibs, boots, gloves) and environment (milking parlors, pens) were collected. Identification of S. Dublin was confirmed with PCR, antimicrobial susceptibility testing was conducted, and genetic profiling done. To evaluate AMR genes, whole genome sequencing was performed using Illumina Miseq platform. Assembled short-read gene sequences were screened using MEGARes database. Results: Clothing from 42 workers and environment were sampled. In total, 37 S. Dublin isolates were identified, some from the same sample. Clothing accounted for approx. 92% of isolates, and approx. 8% were environmental. Whole genome sequencing identified 237 AMR genes. Major gene classes identified across all sample types included beta lactams (48 genes) and multidrug resistance efflux pumps (44 genes). One boot had four fosfomycin resistant genes not previously reported in S. Dublin. Conclusions: This study identified MDR S. Dublin on dairy worker clothing and the environment. Fosfomycin resistance has not been reported in S. Dublin isolated from dairy cattle and warrants further exploration for its public health implications. Examination of the clonal relatedness of clothing and environmental strains will be conducted. The results from this study can help inform disease control practices on dairy farms to limit the spread of drug-resistant zoonotic pathogens from livestock operations. [Description provided by NIOSH]
• Subjects:
  Livestock Occupational Exposure Occupational Health Safety
• Keywords:
  Livestock industry; Animal husbandry workers; Occupational exposure; Antimicrobial resistance; Multidrug resistant organisms; MDROs; Zoonoses; Pathogens
• Publisher:
  Conference of Research Workers in Animal Diseases (CRWAD)
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary ; Abstract or Summary
• Place as Subject:
  Colorado ; OSHA Region 8 ; South Dakota
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• NIOSHTIC Number:
  nn:20070942
• Citation:
  Proceedings of the 101st Conference of Research Workers in Animal Diseases, December 5-8, 2020, virtual event. Champaign, IL: Conference of Research Workers in Animal Diseases (CRWAD), 2020 Dec; :384
• Email:
  njnealon@gmail.com
• Federal Fiscal Year:
  2021
• NORA Priority Area:
  Agriculture, Forestry and Fishing ; Agriculture, Forestry and Fishing
• Performing Organization:
  Colorado State University - Ft. Collins
• Peer Reviewed:
  False
• Start Date:
  20030915
• Source Full Name:
  Proceedings of the 101st Conference of Research Workers in Animal Diseases, December 5-8, 2020, virtual event
• End Date:
  20270914
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/248337/cdc_248337_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]