Sensitive and Direct Detection of Receptor Binding Specificity of Highly Pathogenic Avian Influenza A Virus in Clinical Samples

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Takahashi, Tadanobu ; Kawakami, Tatsuya ; Mizuno, Takashi ; Minami, Akira ; Uchida, Yuko ; Saito, Takehiko ; Matsui, Shigeyuki ; Ogata, Makoto ; Usui, Taichi ; Sriwilaijaroen, Nongluk ; Hiramatsu, Hiroaki ; Suzuki, Yasuo ; Suzuki, Takashi
• Description:
  Influenza A virus (IAV) recognizes two types of N-acetylneuraminic acid (Neu5Ac) by galactose (Gal) linkages, Neu5Acα2,3Gal and Neu5Acα2,6Gal. Avian IAV preferentially binds to Neu5Acα2,3Gal linkage, while human IAV preferentially binds to Neu5Acα2,6Gal linkage, as a virus receptor. Shift in receptor binding specificity of avian IAV from Neu5Acα2,3Gal linkage to Neu5Acα2,6Gal linkage is generally believed to be a critical factor for its transmission ability among humans. Surveillance of this shift of highly pathogenic H5N1 avian IAV (HPAI) is thought to be a very important for prediction and prevention of a catastrophic pandemic of HPAI among humans. In this study, we demonstrated that receptor binding specificity of IAV bound to sialo-glycoconjugates was sensitively detected by quantifying the HA gene with real-time reverse-transcription-PCR. The new assay enabled direct detection of receptor binding specificity of HPAIs in chicken clinical samples including trachea and cloaca swabs in only less than 4 h.
• Subjects:
  Research Article
• Source:
  PLoS One. 2013; 8(10).
• Document Type:
  Journal Article
• Volume:
  8
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:6dd4f8ed2876d93db430aefc8e0997b4e3cae8cca15fcb9739b59c8c17f64879
• Download URL:
  https://stacks.cdc.gov/view/cdc/21330/cdc_21330_DS1.pdf
• File Type:
  [PDF - 685.23 KB ]