Novel Use of µ-PET/CT Imaging to Detect Cardiopulmonary Changes in a Murine Model Following World Trade Center Particular Matter Exposure

Details

• Personal Author:
  Crowley G ; Nolan A ; Oskuei A ; Vaidyanathan S ; Veerappan A ; Wadghiri YZ
• Description:
  Introduction World Trade Center (WTC) particulate matter (PM) exposure is associated with development of airway inflammation, vascular injury and obstructive airways disease. Prior literature on noninvasive quantification and distribution of acute inflammation in the lungs and heart is limited in the context of WTC-PM exposure. FOG-PET offers a valuable tool for detection and monitoring of neutrophilic metabolic activity in vivo. In this study, we aim to characterize the inflammation following WTC-PM exposure within the cardiopulmonary system in a murine model of particulate matter exposure. Methods C57BI6 female mice (> 12 weeks old, Jackson Laboratory, Bar Harbor, ME, USA) underwent intra-tracheal aspiration of 200 µg of PM53 (n=3) or an equal volume physiological-buffered saline (PBS) (n=3). 24 hours after exposure and overnight fasting, anesthesia was induced with 3% isoflurane, followed by maintenance anesthesia with 2% isoflurane at a rate 2 L/min. Supplemental oxygen was provided throughout. 18F-FOG was injected (100-200 µCi of 18F-FOG in 200 µL of PBS) into the tail vein through the Harvard Apparatus PHO 2000 infusion pump at 150 µL/min. µ-PET/CT scanning (Siemens lnveon) of the lung and heart was performed and standardized uptake value (SUV) at a region of interest was recorded (Panel A-C). Results The pre-exposure mean SUV (+/- Standad Error Mean) of the lung was 0.68 +/- 0.02. The mean lung SUV at 24 hours post-exposure was 0.69 +/- 0.02 in the PBS group and 0.84 +/- 0.02 in PM group, p < 0.01 (Panel D). The pre-exposure mean SUV of the heart was 2.867 +/- 0.1453. The mean SUV at 24 hours post-exposure 3.3 +/- 0.06 in PM group, p< 0.05 (Panel E). Overall, the SUV measured at 24 hours was 0.1 and 0.44 units higher in the lung and heart, respectively. Conclusions WTC-PM exposure results in neutrophilic inflammation within the lungs as seen by elevated neutrophil counts in bronchoalveolar lavage samples 24 hours post-exposure. This study further illustrates these findings non-invasively using µ-PET/CT showing a significant elevation in FOG uptake within the lungs and hearts. This is the first study to show the utility of µ-PET/CT in detecting inflammation from particulate matter exposure. Future work will focus on demonstrating these findings at subsequent time points and understanding the underlying pathophysiology. [Description provided by NIOSH]
• Subjects:
  Animals Cohort Studies Firefighters Occupational Health Particulate Matter Safety September 11 Terrorist Attacks
• Keywords:
  Animal Studies Cardiovascular Function Cohort Studies Fire Fighters Imaging Techniques Particulates World Trade Center WTC
• ISSN:
  1073-449X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  199
• NIOSHTIC Number:
  nn:20066279
• Citation:
  Am J Respir Crit Care Med 2019 May; 199(Abstract Issue):A2493
• Email:
  assad.oskuei@nyumc.org
• Federal Fiscal Year:
  2019
• Performing Organization:
  New York University School of Medicine
• Peer Reviewed:
  False
• Start Date:
  20170701
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• Supplement:
  Abstract Issue
• End Date:
  20260630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c949db84bd19f7d672dd87881a311535b03e3008efccfcb59406c2f9d7f9fea72d7f7188b49225083cfe1e82a90bf73bc7cb8bb82ec639997dc4de37212684e9
• Download URL:
  https://stacks.cdc.gov/view/cdc/212757/cdc_212757_DS1.pdf
• File Type:
  [PDF - 156.37 KB ]