“Dip-and-Read” Paper-Based Analytical Devices Using Distance-Based Detection with Color Screening

Details

• Personal Author:
  Citterio D ; Henry CS ; Yamada K
• Description:
  An improved paper-based analytical device (PAD) using color screening to enhance device performance is described. Current detection methods for PADs relying on the distance-based signalling motif can be slow due to the assay time being limited by capillary flow rates that wick fluid through the detection zone. For traditional distance-based detection motifs, analysis can take up to 45 min for a channel length of 5 cm. By using a color screening method, quantification with a distance-based PAD can be achieved in minutes through a "dip-and-read" approach. A colorimetric indicator line deposited onto a paper substrate using inkjet-printing undergoes a concentration-dependent colorimetric response for a given analyte. This color intensity-based response has been converted to a distance-based signal by overlaying a color filter with a continuous color intensity gradient matching the color of the developed indicator line. As a proof-of-concept, Ni quantification in welding fume was performed as a model assay. The results of multiple independent user testing gave mean absolute percentage error and average relative standard deviations of 10.5% and 11.2% respectively, which were an improvement over analysis based on simple visual color comparison with a read guide (12.2%, 14.9%). In addition to the analytical performance comparison, an interference study and a shelf life investigation were performed to further demonstrate practical utility. The developed system demonstrates an alternative detection approach for distance-based PADs enabling fast (approximately 10 min), quantitative, and straightforward assays. [Description provided by NIOSH]
• Subjects:
  Chemistry Chemistry, Analytic Colorimetry Laboratories Nickel Occupational Health Safety Welding
• Keywords:
  Analytical Chemistry Analytical Instruments Analytical Processes Chemical Analysis Chemicals Detectors Direct-reading Methods Filter Materials Filters Fluids Laboratory Techniques Materials Testing Optical Analysis Performance Tests Sample Preparation Sampling Methods Screening Methods Visual Images Welding Fumes

  More +
• ISSN:
  1473-0197
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  18
• Issue:
  10
• NIOSHTIC Number:
  nn:20052783
• Citation:
  Lab Chip 2018 May; 18(10):1485-1493
• Contact Point Address:
  Charles S. Henry, Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, USA
• Email:
  chuck.henry@colostate.edu
• CAS Registry Number:
  Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2018
• Performing Organization:
  Colorado State University, Fort Collins
• Peer Reviewed:
  True
• Start Date:
  20150901
• Source Full Name:
  Lab on a Chip
• End Date:
  20190831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6a3fab4be192438d9166c58327848c6427cc34fdb7bb515b73b878a3548d16e838c93f0efdc644d3cff8ec1f7e0135bb2b62a35d8a3b5e973e12ddb88cfa550e
• Download URL:
  https://stacks.cdc.gov/view/cdc/212258/cdc_212258_DS1.pdf
• File Type:
  [PDF - 2.40 MB ]