Viability of Cultured Primary Human Skin Cells Treated with 1, 6-Hexamethylene Diisocyanate Monomer and Its Oligomer Isocyanurate

Details

• Personal Author:
  Phillips K ; Phillips K
• Description:
  The diisocyanate monomer 1,6-hexamethylene diisocyanate (HDI) and its oligomer HDI isocyanurate are components in sprayed polyurethane coatings. Exposure via the lungs and skin can lead to allergic sensitization and asthma. Research on these compounds has focused on effects of exposure on respiratory and immune cells and variation in gene expression, pathway activation, and mechanisms influencing toxic response. Here, we focused on the toxic effects of HDI monomer and HDI isocyanurate on three types of cultured primary human skin cells, namely keratinocytes, melanocytes, and fibroblasts. To determine the cell-type specific toxicity, we used a luminescent ATP-viability assay. The dose-response data indicated that sensitivity to death varied among the different skin cell types and death by necrosis. The observed variations in toxicity between the HDI monomer and HDI isocyanurate as well as between the cell types may have important implications for developing an adverse effect, for regulatory limits, and for worker safety. [Description provided by NIOSH]
• Subjects:
  Biomarkers Cytotoxins Isocyanates Lung Occupational Health Respiratory System Safety Skin
• Keywords:
  Biological Effects Cytotoxicity Dermal Exposures Diisocyanates Dose Response Exposure Assessment Hexamethylene Diisocyanate Humans Lung Irritants Skin Irritants Toxic Effects

  More +
• Publisher:
  University of North Carolina Chapel Hill
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Thesis ; Thesis
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  1-49
• NIOSHTIC Number:
  nn:20052365
• Citation:
  Chapel Hill, NC: University of North Carolina Chapel Hill, 2017 Jan; :1-49
• CAS Registry Number:
  Hexamethylene diisocyanate (CAS RN 822-06-0) ; Hexamethylene diisocyanate (CAS RN 822-06-0)
• Federal Fiscal Year:
  2017
• Performing Organization:
  University of North Carolina, Chapel Hill
• Peer Reviewed:
  False
• Start Date:
  20050701
• Source Full Name:
  Viability of cultured primary human skin cells treated with 1, 6-hexamethylene diisocyanate monomer and its oligomer isocyanurate
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ee29f5f531689fcb3fc47f6497f867b99cb29cae0011bf952696e8d5865cbdb22ada4fe024dfecb7566b31eda1a0d059fb113032d9ea1fbe09b8b013611d121a
• Download URL:
  https://stacks.cdc.gov/view/cdc/211902/cdc_211902_DS1.pdf
• File Type:
  [PDF - 751.40 KB ]