Details:

Alternative Title:
PLoS Pathog
Publisher's site:
http://dx.doi.org/10.1371/journal.ppat.1003705
Personal Author:
Nandakumar, Subhadra ; Kannanganat, Sunil ; Dobos, Karen M. ; Lucas, Megan ; Spencer, John S. ; ... More +
Nandakumar, Subhadra ; Kannanganat, Sunil ; Dobos, Karen M. ; Lucas, Megan ; Spencer, John S. ; Fang, Sunan ; McDonald, Melissa A. ; Pohl, Jan ; Birkness, Kristin ; Chamcha, Venkateswarlu ; Ramirez, Melissa V. ; Plikaytis, Bonnie B. ; Posey, James E. ; Amara, Rama Rao ; Sable, Suraj B. ; Less -
Description:
Glycosylation is the most abundant post-translational polypeptide chain modification in nature. Although carbohydrate modification of protein antigens from many microbial pathogens constitutes important components of B cell epitopes, the role in T cell immunity is not completely understood. Here, using ELISPOT and polychromatic flow cytometry, we show that O-mannosylation of the adhesin, Apa, of Mycobacterium tuberculosis (Mtb) is crucial for its T cell antigenicity in humans and mice after infection. However, subunit vaccination with both mannosylated and non-mannosylated Apa induced a comparable magnitude and quality of T cell response and imparted similar levels of protection against Mtb challenge in mice. Both forms equally improved waning BCG vaccine-induced protection in elderly mice after subunit boosting. Thus, O-mannosylation of Apa is required for antigenicity but appears to be dispensable for its immunogenicity and protective efficacy in mice. These results have implications for the development of subunit vaccines using post-translationally modified proteins such as glycoproteins against infectious diseases like tuberculosis.
Subject:
[+]
Adhesins, Bacterial
Animals
Antigens, Bacterial
Female
Glycosylation
Humans
Male
Mannose
Mice
Mice, Inbred BALB C
Mycobacterium Bovis
Mycobacterium Tuberculosis
Research Article
T-Lymphocytes
Tuberculosis
Tuberculosis Vaccines

Source:
PLoS Pathog. 2013 ; 9(10).;
Document Type:
Journal Article ;
Funding:
HHSN266200400091C/PHS HHS/United States ; R01 AI071852/AI/NIAID NIH HHS/United States ; U19 AI074073/AI/NIAID NIH HHS/United States ;
HHSN266200400091C/PHS HHS/United States ; R01 AI071852/AI/NIAID NIH HHS/United States ; U19 AI074073/AI/NIAID NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:60528ea20124f110f79d05e4da3f1a7c16e9c3f1278fefb96aa79068c0881931
File Type:

Supporting Files

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