Immunoactivating Peptide P4 Augments Alveolar Macrophage Phagocytosis in Two Diverse Human Populations

Bangert, Mathieu; Wright, Adam K.; Rylance, Jamie; Kelly, Matthew J.; Wright, Angela D.; Carlone, George M.; Sampson, Jacquelyn S.; Rajam, Gowrisankar; Ades, Edwin W.; Kadioglu, Aras; Gordon, Stephen B.

doi:10.1128/AAC.00742-13

i

Immunoactivating Peptide P4 Augments Alveolar Macrophage Phagocytosis in Two Diverse Human Populations

Supporting Files

Jul 01 2013
By Bangert, Mathieu ; Wright, Adam K. ; Rylance, Jamie ; ...

File Language:

English

Details

Alternative Title:

Antimicrob Agents Chemother
Personal Author:

Bangert, Mathieu ; Wright, Adam K. ; Rylance, Jamie ; Kelly, Matthew J. ; Wright, Angela D. ; Carlone, George M. ; Sampson, Jacquelyn S. ; Rajam, Gowrisankar ; Ades, Edwin W. ; Kadioglu, Aras ; Gordon, Stephen B.
Description:

New treatment strategies are urgently needed to overcome early mortality in acute bacterial infections. Previous studies have shown that administration of a novel immunoactivating peptide (P4) alongside passive immunotherapy prevents the onset of septicemia and rescues mice from lethal invasive disease models of pneumococcal pneumonia and sepsis. In this study, using two diverse populations of adult volunteers, we determined whether P4 treatment of human alveolar macrophages would upregulate phagocytic killing of Streptococcus pneumoniae ex vivo. We also measured macrophage intracellular oxidation, cytokine secretion, and surface marker expression following stimulation. Peptide treatment showed enhanced bacterial killing in the absence of nonspecific inflammation, consistent with therapeutic potential. This is the first demonstration of P4 efficacy on ex vivo-derived human lung cells.
Subjects:

Adult Biological Markers Cells, Cultured Cytokines Experimental Therapeutics Female Gene Expression Great Britain Healthy Volunteers Humans Macrophages, Alveolar Male Oligopeptides Oxidation-Reduction Phagocytosis Streptococcus Pneumoniae
Source:

Antimicrob Agents Chemother. 2013; 57(9):4566-4569.
Document Type:

Journal Article
Funding:

101113/Wellcome Trust/United Kingdom ; MC_PC_12017/Medical Research Council/United Kingdom
Place as Subject:

Malawi
Volume:

57
Issue:

9
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:f4ec798141be1256734c0d2794e571c964f293accc82831ed02e2ab93c2e3fc9
Download URL:

https://stacks.cdc.gov/view/cdc/21139/cdc_21139_DS1.pdf
File Type:

[PDF - 439.59 KB ]

File Language:

English

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