Trisaminohexyl Isocyanurate, a Urinary Biomarker of HDI Isocyanurate Exposure

Details

• Personal Author:
  Bodnar W ; Gold, Avram ; Nylander-French LA ; Robbins Z ; Zhang Z
• Description:
  Biological monitoring of occupational exposure to 1,6-hexamethylene diisocyanate (HDI)-containing spray-paints is limited to analysis of metabolites of HDI monomer although polymeric HDI isocyanurate constitutes the predominant inhalation and skin exposure for workers in the automotive paint industry. A novel method using nanoflow ultra-performance liquid chromatography coupled to nano-electrospray ionization tandem mass spectrometry (nano-UPLC-ESI-MS/MS) was developed to quantify trisaminohexyl isocyanurate (TAHI), a hydrolysis product of HDI isocyanurate, in the urine of spray-painters. Analytical and internal standards were synthesized in-house and weighted linear regression calibration curves were generated using spiked control urine from non-exposed persons (0.06-7.98 ug/L; N = 13; w = x- ; r = 0.998). Urine samples collected from 15 exposed workers (N = 111) were subjected to acid hydrolysis and extracted with dichloromethane, then derivatized with acetic anhydride. The derivatized product, trisacetamidohexyl isocyanurate (TAAHI), was analyzed using nano-UPLC-ESI-MS/MS. The protocol was sensitive and specific for analysis of TAHI in the urine of exposed workers with a method detection limit at 0.03 ug/L. TAHI was detected in 33 of 111 urine samples and in 11 of 15 workers. This biomarker for HDI isocyanurate is critical to determine the relative potency and dose-relationships between the monomer and oligomer exposure on the development of diisocyanate induced health effects in future studies. [Description provided by NIOSH]
• Subjects:
  Anhydrides Automobiles Chromatography Energy Metabolism Environmental Exposure Isocyanates Men Methylene Chloride Occupational Health Paint Risk Assessment Risk Factors Safety Skin Urine Women

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• Keywords:
  1,6-Hexamethylene Diisocyanate Author Keywords: Biomarker Automotive Industry Biological Function Dichloromethane Diisocyanates Exposure Exposure Levels Health Effects Hexamethylene Diisocyanate Humans Hydrolysis Isocyanate Isocyanurate Liquid Chromatography-mass Spectrometry Metabolites Monitoring Systems Monomers Paints Risk Factors Skin Exposure Spray Painting Workers

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• ISSN:
  1570-0232
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  117-129
• Volume:
  1076
• NIOSHTIC Number:
  nn:20051011
• Citation:
  J Chromatogr B 2018 Feb; 1076:117-129
• Contact Point Address:
  Leena A. Nylander-French, Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, NC 27599
• Email:
  leena_french@unc.edu
• CAS Registry Number:
  Acetic anhydride (CAS RN 108-24-7) ; Dichloromethane (CAS RN 75-09-2) ; Hexamethylene diisocyanate (CAS RN 822-06-0) ; Hexamethylenediamine (CAS RN 124-09-4) ; Toluene diisocyanate (CAS RN 26471-62-5)
• Federal Fiscal Year:
  2018
• Performing Organization:
  University of North Carolina Chapel Hill
• Peer Reviewed:
  True
• Start Date:
  20140901
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1b06b474a158887ae4988ef502a75e200c9983ec8def8ce9a8813ca9b761e74407153b285ad9d55054b1ff59f2a8868d00e0753b024331f2535675204b559063
• Download URL:
  https://stacks.cdc.gov/view/cdc/211154/cdc_211154_DS1.pdf
• File Type:
  [PDF - 1.63 MB ]