Investigating the Effects of Particulate Matter on House Dust Mite and Ovalbumin Allergic Airway Inflammation in Mice

Details

• Personal Author:
  Castañeda AR ; Pinkerton KE
• Description:
  Particulate matter (PM), a component of air pollution, has been shown to enhance allergen-mediated airway hypersensitivity and inflammation. Surprisingly, exposure to PM during the sensitization to allergen is sufficient to produce immunological changes that result in heightened inflammatory effects upon future allergen exposures (challenge) in the absence of PM. This suggests that PM has the ability to modulate the allergic immune response, thereby acting as an adjuvant by enhancing the immunological memory formed during the adaptive immune response; however, the mechanisms through which this occurs remain elusive. Establishing a reproducible animal model to study the PM-mediated immunotoxicological effects that enhance allergy, may provide insights to understand how air pollution activates the immune system and thereby modulates the pathophysiology of asthma. The basic protocol can be used to study various characteristics of air pollution, such as PM size, source, or chemical composition, to help elucidate how such features may affect the allergic response in a mouse model of asthma. Using a BALB/c model of acute exposure (14 days), mice are first sensitized with allergen and PM, and then subsequently challenged with allergen only. The endpoints of this basic protocol include the assessment of inflammation via cells recovered from broncho-alveolar lavage (BAL), histopathological analysis, gene expression profiles, and protein quantification of inflammatory markers. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution Asthma Asthma, Occupational Hypersensitivity Immune System Laboratories Lung Occupational Health Particulate Matter Respiratory System Safety

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• Keywords:
  Airborne Particles Airborne Particulates Air Pollution Airway Inflammation Allergen Allergens Allergic Reactions Author Keywords: Allergic Mouse Model Cellular Reactions Gene Expression HDM Histopathology House Dust Mite Immune Reaction Immunology Immunotoxins Laboratory Animals Lung Cells Lung Function OVA Ovalbumin Particulate Matter PM Respiratory Function Sensitization

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• ISBN:
  9780471140856
• ISSN:
  1934-9254
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20050942
• Citation:
  Curr Protoc Toxicol 2016 May; (Suppl 68):18.18:1-18.18.18
• Contact Point Address:
  Kent E. Pinkerton, Ph.D., Center for Health and the Environment, Old Davis Road, University of California, Davis, Davis, CA 95616
• Email:
  kepinkerton@ucdavis.edu
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Part Number:
  18.18
• Start Date:
  20010930
• Source Full Name:
  Current Protocols in Toxicology
• Supplement:
  68
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e234fe1d7a5077c739daa17c7f171fa4e4f3f3c5c6221b5c81acab9f258690b6602be363149fe1711f34d03b00cf186b1045ae2c0039f4432581695c93faf4e6
• Download URL:
  https://stacks.cdc.gov/view/cdc/211119/cdc_211119_DS1.pdf
• File Type:
  [PDF - 196.07 KB ]