Details:

Alternative Title:
Curr Protoc Toxicol
Personal Author:
Castañeda, Alejandro R. ; Pinkerton, Kent E.
Castañeda, Alejandro R. ; Pinkerton, Kent E. Less -
Description:
Particulate matter (PM), a component of air pollution, has been shown to enhance allergen-mediated airway hypersensitivity and inflammation. Surprisingly, exposure to PM during the sensitization to allergen is sufficient to produce immunological changes that result in heightened inflammatory effects upon future allergen exposures (challenge) in the absence of PM. This suggests that PM has the ability to modulate the allergic immune response, thereby acting as an adjuvant by enhancing the immunological memory formed during the adaptive immune response; however, the mechanisms through which this occurs remain elusive. Establishing a reproducible animal model to study the PM-mediated immunotoxicological effects that enhance allergy, may provide insights to understand how air pollution activates the immune system and thereby modulates the pathophysiology of asthma. The basic protocol can be used to study various characteristics of air pollution, such as PM size, source, or chemical composition, to help elucidate how such features may affect the allergic response in a mouse model of asthma. Using a BALB/c model of acute exposure (14 days), mice are first sensitized with allergen and PM, and then subsequently challenged with allergen only. The endpoints of this basic protocol include the assessment of inflammation via cells recovered from broncho-alveolar lavage (BAL), histopathological analysis, gene expression profiles, and protein quantification of inflammatory markers. © 2016 by John Wiley & Sons, Inc.
Subjects:
[+]
Air Pollution Airway Inflammation Allergen Allergic Mouse Model Article House Dust Mite (HDM) Ovalbumin (OVA) Particulate Matter (PM)
Source:
Curr Protoc Toxicol. 68:18.18.1-18.18.18.
Pubmed ID:
27145110
Pubmed Central ID:
PMC4988128
Document Type:
Journal Article
Funding:
U50 OH007550/OH/NIOSH CDC HHS/United States ; P51 OD011107/OD/NIH HHS/United States ; P30 ES023513/ES/NIEHS NIH HHS/United States ; U54 OH007550/OH/NIOSH CDC HHS/United States ; T32 GM099608/GM/NIGMS NIH HHS/United States
U50 OH007550/OH/NIOSH CDC HHS/United States ; P51 OD011107/OD/NIH HHS/United States ; P30 ES023513/ES/NIEHS NIH HHS/United States ; U54 OH007550/OH/NIOSH CDC HHS/United States ; T32 GM099608/GM/NIGMS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:c40ff95fc7c083cba60349aa605c22e68dfb35c74b27ff7a74461cb004061ff1
Download URL:
https://stacks.cdc.gov/view/cdc/40932/cdc_40932_DS1.pdf
File Type:

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