A Role for B Cells in Organic Dust Induced Lung Inflammation

Details

• Personal Author:
  Duryee MJ ; Mikuls TR ; Nelson AJ ; Poole JA ; Romberger DJ ; Thiele GM ; Warren KJ ; West WW ; Wyatt TA
• Description:
  BACKGROUND: Agriculture organic dust exposures induce lung disease with lymphoid aggregates comprised of both T and B cells. The precise role of B cells in mediating lung inflammation is unknown, yet might be relevant given the emerging role of B cells in obstructive pulmonary disease and associated autoimmunity. METHODS: Using an established animal model, C57BL/6 wild-type (WT) and B-cell receptor (BCR) knock-out (KO) mice were repetitively treated with intranasal inhalation of swine confinement organic dust extract (ODE) daily for 3 weeks and lavage fluid, lung tissues, and serum were collected. RESULTS: ODE-induced neutrophil influx in lavage fluid was not reduced in BCR KO animals, but there was reduction in TNF-alpha, IL-6, CXCL1, and CXCL2 release. ODE-induced lymphoid aggregates failed to develop in BCR KO mice. There was a decrease in ODE-induced lung tissue CD11c+CD11b+ exudative macrophages and compensatory increase in CD8+ T cells in lavage fluid of BCR KO animals. Compared to saline, there was an expansion of conventional B2-, innate B1 (CD19+CD11b+CD5+/-)-, and memory (CD19+CD273+/-CD73+/-) B cells following ODE exposure in WT mice. Autoreactive responses including serum IgG anti-citrullinated protein antibody (ACPA) and anti-malondialdehyde-acetaldehyde (MAA) autoantibodies were increased in ODE treated WT mice as compared to saline control. B cells and serum immunoglobulins were not detected in BCR KO animals. CONCLUSIONS: Lung tissue staining for citrullinated and MAA modified proteins were increased in ODE-treated WT animals, but not BCR KO mice. These studies show that agriculture organic dust induced lung inflammation is dependent upon B cells, and dust exposure induces an autoreactive response. [Description provided by NIOSH]
• Subjects:
  Agriculture Animals Antibodies Dust Immune System Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Author Keywords: ACPA Autoantibody Immune Reaction Immunoglobulin Immunology Inflammation Lung Disease MAA Adduct Organic Dust Organic Dusts Particulates Proteins Respiratory Disease

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• ISSN:
  1465-9921
• Document Type:
  Text
• Funding:
  Cooperative Agreement ; Grant
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  18
• NIOSHTIC Number:
  nn:20050744
• Citation:
  Respir Res 2017 Dec; 18:214
• Contact Point Address:
  Jill A. Poole, Pulmonary, Critical Care, Sleep & Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center (UNMC), 985990 Nebraska Medical Center, Omaha, NE 68198-5990
• Email:
  japoole@unmc.edu
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center - Omaha
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Respiratory Research
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:67da2296bf08aff83fb2ba941380b38061410534f5e8625a602520b44b21f30fb9f7c677dda7202fd4794536c4ca55e289f1e8bafe28d7357fa78467d2b133df
• Download URL:
  https://stacks.cdc.gov/view/cdc/210969/cdc_210969_DS1.pdf
• File Type:
  [PDF - 2.37 MB ]