Assessment of Reactive Oxygen Species Generated by Electronic Cigarettes Using Acellular and Cellular Approaches

Details

• Personal Author:
  Bello D ; Demokritou P ; Leonard, Stephen S. ; Morris AM ; Qian Y ; Shaffer J ; Sisler JD ; Zhang Y ; Zhao J
• Description:
  Electronic cigarettes (e-cigs) have fast increased in popularity but the physico-chemical properties and toxicity of the generated emission remain unclear. Reactive oxygen species (ROS) are likely present in e-cig emission and can play an important role in e-cig toxicity. However, e-cig ROS generation is poorly documented. Here, we generated e-cig exposures using a recently developed versatile exposure platform and performed systematic ROS characterization on e-cig emissions using complementary acellular and cellular techniques: 1) a novel acellular Trolox-based mass spectrometry method for total ROS and hydrogen peroxide (H2O2) detection, 2) electron spin resonance (ESR) for hydroxyl radical detection in an acellular and cellular systems and 3) in vitro ROS detection in small airway epithelial cells (SAEC) using the dihydroethidium (DHE) assay. Findings confirm ROS generation in cellular and acellular systems and is highly dependent on the e-cig brand, flavor, puffing pattern and voltage. Trolox method detected a total of 1.2-8.9 nmol H2O2eq./puff; H2O2accounted for 12-68% of total ROS. SAEC cells exposed to e-cig emissions generated up to eight times more ROS compared to control. The dependency of e-cig emission profile on e-cig features and operational parameters should be taken into consideration in toxicological studies. [Description provided by NIOSH]
• Subjects:
  Chemistry Electrical Equipment And Supplies Environmental Exposure Hazardous Substances Nicotine Occupational Health Oxygen Risk Assessment Risk Factors Safety Smokers Tobacco Products Toxicology

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• Keywords:
  Author Keywords: Electronic Cigarette Cellular Function Chemical Properties Cigarette Smoke Cigarette Smoking Electronic Devices Emission Sources Exposure Levels Hydrogen Peroxide Mass Spectrometry Oxidative Stress Physical Properties Reactive Oxygen Species Risk Factors Toxins

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• ISSN:
  0304-3894
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  344
• NIOSHTIC Number:
  nn:20050654
• Citation:
  J Hazard Mater 2018 Feb; 344:549-557
• Contact Point Address:
  Dhimiter Belloa, Department of Environmental Health, Harvard Schoolof Public Health, Boston, MA, 02115
• Email:
  Dhimiter_Bello@uml.edu
• CAS Registry Number:
  Hydrogen peroxide (CAS RN 7722-84-1) ; Oxygen (CAS RN 7782-44-7)
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Construction
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Hazardous Materials
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:67e31897eb314b4bb61f5ccac238da2e3dc33c5e1ddc36cb3185925d21f6ec7c39fac34b61486029d5ad87fa70c7f1199c04f1e8c3268721bd54d6e9fdd8646e
• Download URL:
  https://stacks.cdc.gov/view/cdc/210909/cdc_210909_DS1.pdf
• File Type:
  [PDF - 1.40 MB ]