Nanoparticle-Based Electrochemical Immunosensor for Detection of Phosphorylated Acetylcholinesterase Adduct: An Exposure Biomarker of Organophosphate Pesticides and Nerve Agents

Details

• Personal Author:
  Barry R ; Gassman PL ; Lin Y ; Liu G ; Petersen C ; Timchalk C ; Wang J
• Description:
  Simple, rapid and sensitive sensors are needed to evaluate chemical nerve agent exposures. To detect sub-clinical exposures, sensor development is focused on the detection of adducts on target proteins. Specifically the development of an electrochemical immunosensor for determination of phosphorylated cholinesterase (OP-ChE) adduct biomarkers using quantum dot (QD) labels. Paraoxon was used as a model organophosphrous compound to prepare phosphorylated acetylcholinesterase (AChE) to demonstrate the proof of principle. A nanoparticle-based electrochemical immunosensor has been developed for the detection of OP-ChE. Zirconia nanoparticles (ZrO2 NPs) were used as selective sorbents to capture the phosphorylated AChE adduct, and quantum dots (ZnS@CdS, QDs) were used as tags to label monoclonal anti-AChE antibody to track the immunorecognition events. The sandwich-like immunoreactions were performed among the ZrO2 NPs, which were pre-coated on a screen printed electrode (SPE) by electrodeposition, phosphorylated AChE and QD-anti-AChE. The captured QD tags were determined on the SPE by electrochemical stripping analysis of its metallic component (cadmium) after an acid-dissolution step. The paraoxon-AChE adduct was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and the binding affinity of anti-AChE to the paraoxon-AChE was validated with an enzyme-linked immunosorbent assay. The parameters (e.g., amount of ZrO2 NP, QD-anti-AChE concentration,) that govern the electrochemical response of immunosensors were optimized. The voltammetric response of the immunosensor is highly linear over the range of 10 pM to 10 nM paraoxon-AChE, and the limit of detection is estimated to be 8 pM. This new nanoparticle-based electrochemical immunosensor thus provides a sensitive and quantitative tool for biomonitoring exposure to OP insecticides and chemical nerve agents. [Description provided by NIOSH]
• Subjects:
  Agriculture Biomarkers Chemical Warfare Agents Chemistry Environmental Exposure Hypersensitivity Immune System Insecticides Nervous System Occupational Health Organophosphates Safety

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• Keywords:
  Agricultural-chemicals Biological-effects Chemical-analysis Chemical-hypersensitivity Chemical-properties Chemical-reactions Chemical-synthesis Chemical-warfare-agents Electrochemical-analysis Electrochemical-properties Electrochemical-reactions Exposure Levels Immune-reaction Immunochemistry Models Nanoparticles Nanotechnology Nerve-function Nervous-system-function Organo-phosphorus-compounds Organo-phosphorus-pesticides Parasympathetic-nervous-system Pesticides-and-agricultural-chemicals Proteins Qualitative-analysis Sensors

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• ISSN:
  1096-6080
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  102
• Issue:
  1
• NIOSHTIC Number:
  nn:20050541
• Citation:
  Toxicologist 2008 Mar; 102(1):138
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  False
• Start Date:
  20060901
• Source Full Name:
  The Toxicologist
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9aa2bd1054f1db3f8744308a2a8995bcb3b3c26e42fa997d4a45b95931530df9387582c2a6ac3679ab4bb3ea84975705e4e49a2ead729dcfa6da43e9ac0d97e3
• Download URL:
  https://stacks.cdc.gov/view/cdc/210848/cdc_210848_DS1.pdf
• File Type:
  [PDF - 1.36 MB ]